Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) of two AML cell lines HNT34 and UCSD/AML1 and one primary sample. EVI-1 is immunoprecipitated and the nearby chromatin-bound proteins are identified.
To identify the target genes of Evi-1 in hematopoietic stem cells (HSCs), we carried out genome-wide transcriptional analysis using wild-type and Evi-1-deleted HSCs. Experiment Overall Design: Lineage(-), Sca-1(+), c-Kit(+) cells derived from Evi-1(flox/flox) mice were transduced with GFP or Cre-GFP...
To identify the target genes of Evi-1 in hematopoietic stem cells (HSCs), we carried out genome-wide transcriptional analysis using wild-type and Evi-1-deleted HSCs.
Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) of two AML cell lines HNT34 and UCSD/AML1 and one primary sample. EVI-1 is immunoprecipitated and the nearby chromatin-bound proteins are identified.
RUNX1 is a frequent target of translocations in acute myeloid leukemia whereby its DNA binding domain fuses to different epigenetic regulators. To assess how different RUNX1 fusion proteins interact with the epigenome we compared the global binding patterns and the chromatin landscape of t(8;21) and...
