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TranscriptomicsEWS siRNA knock-down in HeLa cell line
HeLa cell line was trasfected by a EWS siRNA oligo causing knock-down of EWS or a siRNA scrambled oligo Three biological replicates were labeled in direct and dye-swap microarray experiments and hybridized onto an Agilent custom splicing-sensitive microarray platform.
ORGANISM(S): Homo sapiens 
2011-09-19 | E-GEOD-22929 | biostudies-arrayexpress
TranscriptomicsRNA immunoprecipitation (RIP)-Chip analysis for EWS-bound mRNA
Although EWS/FLI-1 fusion protein is responsible for most EwingM-bM-^@M-^Ys sarcoma family tumors (ESFT), the function of native EWS remains largely unknown. Here, we first showed that EWS repressed protein expression in a tethering assay. mRNAs bound to EWS were determined by RNA-immunoprecipitatio...
ORGANISM(S): Homo sapiens 
2012-01-16 | E-GEOD-29313 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Ewing sarcoma samples and EWS-FLI-1 inhibited Ewing sarcoma cell lines
The cellular origin of Ewing tumor (ET), a tumor of bone or soft tissues characterized by specific fusions between EWS and ETS genes, is highly debated. Through gene expression analysis comparing ETs with a variety of normal tissues, we show that the profiles of different EWS-FLI1-silenced Ewing cel...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7007 | biostudies-arrayexpress
TranscriptomicsDifferential gene expression in GBS6 cells after EWS-POU5F1 knockdown
Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone sarcoma carrying translocation t(6;22)(p21;q12). The translocation resulted in a gene fusion between EWS and POU5F1. Gene expression analysis of t(6;22) undifferenti...
ORGANISM(S): Homo sapiens 
2009-05-31 | E-GEOD-12320 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human NOR1- and EWS/NOR1-overexpressing 293 cells
To examine the differences between NOR1 and its fusion gene product EWS/NOR1, we compared the gene expression profiles of NOR1- and EWS/NOR1-overexpressing 293 cells. Experiment Overall Design: We established 293-tet-On clones stably transfected NOR1- or EWS/NOR1-expressing plasmid. Total RNA was co...
ORGANISM(S): Homo sapiens 
2009-01-14 | E-GEOD-11185 | biostudies-arrayexpress
TranscriptomicsEffect of EWS/FLI1 and DAX1 silencing on the gene expression profile of the A673 Ewing cell line
The molecular hallmark of the Ewing family of tumors is the presence of balanced chromosomal translocations leading to the formation of chimerical transcription factors (i.e. EWS/FLI1) that play a pivotal role in the pathogenesis of Ewing tumors by deregulating gene expression. We have recently demo...
ORGANISM(S): Homo sapiens 
2012-02-23 | E-GEOD-36007 | biostudies-arrayexpress
TranscriptomicsLow-dose actinomycin D preferentially disrupts EWS-FLI1DNA binding.
Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds that disrupt the binding of EWS-FLI1 to its cognate DNA targets. A number of DNA...
ORGANISM(S): Homo sapiens 
2013-08-26 | E-GEOD-45414 | biostudies-arrayexpress
TranscriptomicsEWS-WT1 Oncogene Activates a Neuronal Reprogramming Factor ASCL1 and Mediates Partial Neural Differentiation
A chromosomal translocation fusion gene product EWS-WT1 is the defining genetic event in Desmoplastic Small Round Cell Tumor (DSRCT), a rare but aggressive tumor with a high rate of mortality. EWS-WT1 oncogene acts as an aberrant transcription factor that drives tumorigenesis, but the mechanism by w...
ORGANISM(S): Mus musculus 
2014-09-11 | E-GEOD-53301 | biostudies-arrayexpress
TranscriptomicsTargeting the EWS/ETS transcriptional program by BET bromodomain inhibition in Ewing sarcoma
Ewing sarcomas (ES) are highly malignant, osteolytic bone or soft tissue tumors, which are characterized by early metastasis into lung and bone. Genetically, ES are defined by balanced chromosomal EWS/ETS translocations, which give rise to chimeric proteins (EWS-ETS) that generate an oncogenic trans...
ORGANISM(S): Homo sapiens 
2015-12-08 | E-GEOD-72673 | biostudies-arrayexpress
TranscriptomicsRegulation of FAS Exon Definition and Apoptosis by the Ewing Sarcoma Protein
The EwingM-bM-^@M-^Ys sarcoma protein EWS belongs to the TET family (FUS/TLS, EWS, TAF15) of RNA and DNA binding proteins, implicated in DNA transcription, pre-mRNA splicing and maintenance of genomic integrity. Translocations of these genes are characteristic of particular neoplasias, including Ewi...
ORGANISM(S): Homo sapiens 
2014-05-21 | E-GEOD-48065 | biostudies-arrayexpress
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