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To identify acetylation-dependent posttranslational modifications (PTMs) of G6PD, site-specifically acetylated and Flag-tagged G6PD was expressed in HEK293T cells by genetically encoding the incorporation of acetylated lysine in response to an in-frame TAG stop codon. K403-acetylated G6PD (sample) a...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD041775 | Pride
check the effect of over expression and down regulation of this clade of TFs Experiment Overall Design: each line have two replicate
ORGANISM(S): Arabidopsis thaliana 
GC-MS single quad metabolomes of fecal samples from 101 individual animals from 25 mammalian species, in collaboration with a zoo. Includes fecal samples, dietary samples, and quality control samples. See linked datasets: MSV000086131, https://www.ncbi.nlm.nih.gov/bioproject/PRJNA693262/ Reference:...
ORGANISM(S): Mammalia (ncbitaxon:40674) 
2019-05-26 | MSV000083859 | GNPS
CD24 is a potential oncogene reported to be overexpressed in a large variety of human malignancies. We have shown that CD24 is overexpressed in 90% of colorectal tumors at a fairly early stage in the multistep process of carcinogenesis. Anti-CD24 monoclonal antibodies (mAb) induce a significant grow...
ORGANISM(S): Homo sapiens 
gp130Act cDNA was PCR-amplified and inserted into a plasmid containing the 12.4-kb Villin promoter. The 15.7-kb expression cassette was excised by PmeI digestion, purified, and injected into fertilized C57BL/6 oocytes to obtain founder mice, two of which transmitted the gp130Act transgene. Small int...
ORGANISM(S): Mus musculus 
Site-specifically acetylated G6PD expressed in bacteria or cultured mammalian cells by genetically encoding the incorporation of acetylated lysine. Purified G6PD (expressed in E. coli) or immunopurified G6PD (expressed in HEK293T cells) were subjected to LC-MS/MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD044906 | Pride
Alternative mRNA splicing is a major mechanism for gene regulation and transcriptome diversity. Despite the extent of the phenomenon, the regulation and specificity of the splicing machinery are only partially understood. Adenosine-to-inosine (A-to-I) RNA editing of pre-mRNA by ADAR enzymes has been...
ORGANISM(S): Homo sapiens 
HEK293T cells, stably transfected with pyrrolysine UAG suppressor tRNA and aminoacyl tRNA synthetase, for the co-translational incorporation of Boc-protected lysine.
ORGANISM(S): Homo sapiens (Human) 
2020-02-24 | PXD016728 | Pride
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