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ProteomicsProteomics of HeLa cells starved and treated with YWF or leptomycin B
HeLa cells labeled with SILAC and were amino acids starved and treated with YWF, leptomycin B, MG132 or chloroquine. Cells were lysed with a 8M Urea buffer and proteins were reduced and alkylated. Equal amount of proteins of different labels were mixed and were digested with trypsin and resulting pe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-01 | MSV000092564 | MassIVE
ProteomicsComparison of Drosophila melanogaster adult and embryo proteome
Embryo and adult flies infected with Wolbachia pipientis or cured were lysed in a SDS containing buffer and digested with trypsin using in gel digestion. The resulting peptides were injected on a Sciex Triple-TOF 6600 in SWATH-MS mode. The data were analysed using Spectronaut 10 using the spectral l...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-08-10 | MSV000082812 | MassIVE
ProteomicsFBXO7 Binds to and Increases the Expression of the p105 Subunit of NF-kB
Cell lysate from HAP1 cells were separated on several columns, the proteins were digested with trypsin and analysed on a Q Exactive HF-X instrument.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-08-05 | MSV000085912 | MassIVE
ProteomicsProteasome composition analysis
Proteasome complexes diversity was studied by AP-MS approaches.
ORGANISM(S): Homo sapiens (Human) 
2016-07-08 | PXD001043 | Pride
Human Proteasome 
ProteomicsRegulation of proteome by the proteasome upon ER stress
HeLa cells were with Tunicamycin or Thapsigargin for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-04-30 | MSV000083736 | MassIVE
ProteomicsProteomics analysis of HeLa cells starved with or without MG132
HeLa cells were amino acid starved for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-30 | MSV000083644 | MassIVE
ProteomicsAnalysis of heat-shock treated Drosophila melanogaster embryos by SWATH-MS
Protein expression levels of Drosophila melanogaster embryos were monitored by SWATH-MS after heat-shock treatments.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-12-23 | PXD004753 | Pride
Embryo Swath Drosophila melanogaster 
ProteomicsCharacterization of the tomato fruit pericarp proteome
In this work, we present an extended characterization of the proteome of the tomato pericarp in its ripe red stage. An extensive fractionation of peptides through high pH reverse phase was performed associated to LC-MS/MS analysis on a Sciex Triple-TOF 6600.
ORGANISM(S): Solanum lycopersicum 
2016-12-22 | PXD004932 | Pride
Tomato  solanum lycopersicum  pericarp  mass-spectrometry  triple-tof 6600 
ProteomicsDrosophila melanogaster proteome dynamics during embryogenesis - 1
In this study we use a combination of proteomics Label-Free quantification methods to monitor protein expression changes over a time course of more than 20 hours of embryo development in Drosophila melanogaster.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-04-11 | PXD003178 | Pride
Drosophila melanogaster  label-free quantification  swath 
ProteomicsProteomics of treatment of Arabidopsis thaliana with peptides
Plants were treated with a cPEP for luciferase (PEP Luc) or a scrambled version of this peptide (4 biological replicates each). Leaves were snap frozen in liquid nitrogen and grounded into powder. The powder was resuspended in tris 50 mM and 5% SDS, sonicated and centrifuged at 14,000 g for 10 min. ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000090305 | MassIVE
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