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TranscriptomicsHalobacterium NRC-1 oxygen, light and pertubations
This SuperSeries is composed of the following subset Series: GSE7714: Halobacterium NRC-1 oxygen, light and phr1 perturbation GSE7715: Halobacterium NRC-1 oxygen, light and ura3 perturbation GSE7716: Halobacterium NRC-1 oxygen, light and phr1/phr2 perturbation GSE7717: Halobacterium NRC-1 oxygen, li...
ORGANISM(S): Halobacterium sp. NRC-1 
2008-01-02 | E-GEOD-7740 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 General transcription factor perturbation
Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7713 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1, H2O2 Stress Response
50mM H2O2 was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of H2O2 for 30 minutes, cultures were spun down and pellets were resuspended in same volume of GN101 media. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7610 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen and light perturbation
A 50ml culture of Halobacterium NRC-1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7729 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr1 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr1 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7726 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and kinA2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of kinA2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard cle...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7727 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr8 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr8 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7728 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and vng2334C perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of vng2334c was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard ...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7733 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and zim perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of zim was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7734 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and htr2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7731 | biostudies-arrayexpress
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