Halobacterium NRC-1 General transcription factor perturbation

ABSTRACT: Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent Bioanalyzer and with Oligo Microarrays were fabricated at the Institute for Systems Biology Microarray Facility. The arrays contain 4 spots per unique 70-mer oligonucleotides for each of 2400 non-redundant genes in Halobacterium NRC-1 Labeling, hybridization and washing have been previously described (Baliga et al. 2002) with 10 μg of RNA from the sample and reference. RNA from the final time point of a replicate experiment was used as the reference. . Bias in dye incorporation was account

ORGANISM(S): Halobacterium sp. NRC-1

SUBMITTER: Nitin Baliga 

PROVIDER: E-GEOD-7713 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress