Halobacterium NRC-1, EMS Stress Response

ABSTRACT: 100mM EMS was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of EMS for 30 minutes, cultures were spun down and pellets were re-suspended in same volume of GN101 media. Cultures were then harvested by centrifugation and pellets were snap frozen on a dry-ice ethanol bath. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. 16 samples (8 samples from EMS perturbed and 8 non-perturbed controls) were analyzed on replicate arrays (dye-flips) all against the same standard reference sample.

ORGANISM(S): Halobacterium sp. NRC-1

SUBMITTER: Amardeep Kaur 

PROVIDER: E-GEOD-7609 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress