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Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Protein identification was carried out by searching against a joint Human + Yeast Swissprot database (Uniprot release 57.3 May 2009, 26885 entries), in order to increase statistical power of peptide identification, which was supplemented with porcine trypsin, and using SEQUEST algorithm (Bioworks 3....
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-05-11 | PXD000325 | Pride
Emerging new evidence highlights the importance of prolonged daily fasting periods for the health and survival benefits of calorie restriction (CR) and time-restricted feeding (TRF) in male mice; however, little is known about the impact of these feeding regimens in females. We placed 14-month-old f...
2024-05-14 | MTBLS790 | MetaboLights
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