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ProteomicsPyrophosphoproteomics in HCT116 wildtype vs PPIP5K1/2 knockout
In vitro, both 5PP-InsP5 (IP7) and 1,5(PP)2InsP4 (IP8) can pyrophosphorylate peptides and proteins. It is unclear, which inositol pyrophosphate species is responsible for installing endogenous pyrophosphorylation in cells. In order to test this, we used HCT116 cells deficient in IP8 synthesis. Both ...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD048031 | JPOST Repository
ProteomicsPyrophosphoproteomics on NOLC1 / TCOF1 / IWS1 pulldown samples
Known pyrophosphorylation substrates NOLC1, TCOF1, IWS1 were overexpressed in IP6K1-/- double KO HEK293T cells, along with active WT or kinase-dead IP6K1 i.e. background high or low in inositol pyrophosphates. the three proteins were pulled down via their SFB-tag and submitted to a pyrophosphoproteo...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD038962 | JPOST Repository
ProteomicsPyrophosphoproteomics: extensive protein pyrophosphorylation revealed in human cell lines
Reversible protein phosphorylation is a central signaling mechanism in eukaryotic cells. While the identification of canonical phosphorylation sites using mass-spectrometry (MS) based proteomics has become routine, annotation of non-canonical phosphorylation has remained a challenge. Here, we report...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD038963 | JPOST Repository
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