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ProteomicsLabel-free shotgun proteomics of M. hyopneumoniae and M. feriruminatoris
Mycoplasmas are wall-less parasitic bacteria possessing extremely small genomes. Despite this, growth rates differ among mycoplasma species with doubling times ranging from only 0.5h to 16h. Here, we analyzed the whole proteomes of M. hyopneumoniae and M. feriruminatoris and performed a comparative ...
ORGANISM(S): Mycoplasma hyopneumoniae (strain 232) Mycoplasma feriruminatoris 
2021-08-23 | PXD025422 | Pride
M. hyopneumoniae M. feriruminatoris 
MetabolomicsImproving global feature detectabilities through scan range splitting for untargeted metabolomics by high-performance liquid chromatography-Orbitrap mass spectrometry
Untargeted metabolomics aims at obtaining quantitative information on the highest possible number of low-molecular biomolecules present in a biological sample. Rather small changes in mass spectrometric spectrum acquisition parameters may have a significant influence on the detectabilities of metabo...
2016-06-09 | MTBLS233 | MetaboLights
MetabolomicsStable Isotope–Assisted Plant Metabolomics: Combination of Global and Tracer-Based Labeling for Enhanced Untargeted Profiling and Compound Annotation
Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-b...
2020-02-09 | MTBLS1217 | MetaboLights
MetabolomicsMetabolome analysis via an HPLC-ESI-MS-based experimental and computational pipeline for chronic nephron toxicity profiling
Untargeted metabolomics has the potential to improve the predictivity of in vitro toxicity models and therefore may aid the reduction of expensive and laborious animal models. Here we describe a chronic nephrotoxicity study conducted on a human renal proximal tubular epithelial cell line (RPTEC/TERT...
2015-09-07 | MTBLS140 | MetaboLights
UnknownInvestigation of Motivational Interviewing and Prevention Consults to Achieve Cardiovascular Targets (IMPACT) trial.
Not available
S-EPMC6276124 | biostudies-literature
MetabolomicsMetabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorecta...
2021-10-12 | MTBLS2130 | MetaboLights
ProteomicsProteome wide measurements of protein half-lives in Mycoplasma pneumoniae
We obtained proteome wide measurements of protein half-lives in M. pneumoniae by performing pulse-chase experiments following SILAC methods. We estimated degradation rates of individual proteins by measuring the increase in light protein compared to heavy protein labeling along the growth curve.
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2020-11-24 | PXD016386 | Pride
Degradation rates Protein half-life Silac 
UnknownPlatelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis.
Not available
S-EPMC6905432 | biostudies-literature
ProteomicsCopper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations
Changes in leaf soluble proteome were explored in 3-month-old plants of Cu-tolerant (metallicolous, M) and sensitive (non-metallicolous, NM) Agrostis capillaris L. populations exposed to increasing Cu concentrations (1-50 µM). Aims of the study were to investigate (1) differential protein expression...
ORGANISM(S): Agrostis capillaris (Colonial bentgrass) (Agrostis tenuis) 
2016-04-12 | PXD001930 | Pride
Plant proteomics  lc-ms/ms  agrostis capillaris  cu-tolerance  photosynthesis  heat-shock protein 
ProteomicsTransitions in the proteome and phospho-proteome during Xenopus laevis development.
Vertebrate development from an egg to a complex multi-tissue million-cell organism is supported by multiple phases of genome-scale remodeling of the repertoire of proteins and their post-translational modifications, yet so far we know little about these phases. In this paper we present comprehensive...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2025-07-14 | PXD060481 | Pride
Tmt Development Eclipse Lumos Xenopus laevis 
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