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MG1655 Flagellar Regulators RNA-seq
This study examined the genes under the control of FlhDC and sigmaF in E. coli. Keywords: wild-type, deletion and overexepression strains Under defined steady-state growth condition, we used two different genetic approaches to alter the modulator concentration in cells; (1) moderately expressing Fl...
ORGANISM(S): Escherichia coli 
This experiment contains RNA-seq data for a motile derivative of MG1655 and isogenic strains with deletions of each flagellar regulator: flhD, flhC, and fliA. All strains were grown at 37 degrees C in LB. After RNA purification, ribosomal RNA was removed using RiboZero and a library was made of rema...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
We mapped the genome-wide binding of the flagellar regulators FlhD, FlhC, and FliA in FLAG-tagged derivatives of E. coli K-12 MG1655 using ChIP coupled with deep sequencing (ChIP-seq). We identify new binding sites for each factor.
ORGANISM(S): Escherichia coli 
In the syntrophic interaction between fermentative bacteria (Pelotomaculum thermopropionicum) and methanogenic archaea (methanogens: Methanothemobacter thermautotrophicus), reducing equivalents (e.g., H2) produced by fermentative bacteria should efficiently be consumed by methanogens in order for th...
ORGANISM(S): Methanobacterium thermoautotrophicum 
MG1655 flagellar regulator ChIP-seq (FlhD, FlhC, FliA)
Using electron cryo-tomography (ECT), we show that, in three bacterial species, the flagellar motor disassembly process results in stable outer-membrane-embedded sub-complexes. These sub-complexes consist of the periplasmic embellished P- and L-rings, and bend the membrane inward while it remains ap...
ORGANISM(S): Shewanella oneidensis (strain MR-1) 
2019-05-21 | PXD012554 | Pride
Transcriptional organization and regulation of the Pseudomonas putida flagellar system
Flagellar mutants have reduced pilus synthesis in Caulobacter crescentus
Here we demonstrate by experimental evolution and genetics the rapid and repeatable evolutionary re-wiring of regulatory pathways, where the cellular nitrogen regulatory system acquired the ability to “cross-talk” with the flagellar regulon. This rewiring restored flagella to aflagellate strains of ...
ORGANISM(S): Pseudomonas fluroscens 
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