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ProteomicsA plant Cajal body proteome reveals kingdom-specific components
Cajal bodies from Nicotiana benthamiana were isolated using fluorescence-activated particle sorting (FAPS) and subjected to LC-MS/MS for comprehensive proteomic profiling. In parallel, TurboID-based proximity labeling was performed using Cajal body-resident proteins to identify their proximal proteo...
ORGANISM(S): Nicotiana benthamiana 
2026-09-21 | PXD065938 | Pride
Nicotiana benthamiana Proximity labeling Cajal body Fluorescence-activated particle sorting (faps) Lc-ms/ms 
ProteomicsMass Spectrometry of FAPS sorted ELVAs by an alternative sorting strategy
We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together w...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-20 | PXD047716 | Pride
Elvas oocyte faps 
ProteomicsLC-MS of FAPS-sorted ELVAs from mouse oocytes
We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together w...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-20 | PXD042768 | Pride
Mouse Oocyte Vesicular assemblies Faps 
ProteomicsQuantitative proteomic analysis of p62-body components isolated by FAPS method.
We developed a method using fluorescence-activated particle sorting (FAPS) to purify p62-bodies efficiently and identified several p62-body components, including known not only autophagy substrates and receptors.
ORGANISM(S): Homo Sapiens (human) 
2023-04-24 | PXD040119 | JPOST Repository
ProteomicsProteomic analysis of p62-body components isolated by FAPS method.
Here, we developed a method using fluorescence-activated particle sorting (FAPS) to purify p62-bodies efficiently and identified several p62-body components, including known not only autophagy substrates and receptors, but also subunits of supramolecular protein complexes such as vault, a large cyto...
ORGANISM(S): Homo Sapiens (human) 
2023-04-24 | PXD037012 | JPOST Repository
TranscriptomicsPurification-sequencing of RNA super-assemblies.
C. elegans oogenesis arrest offers a unique and biologically relevant opportunity to investigate how condensation reorganizes the transcriptome in the context of cellular adaptation to quiescence. To investigate how RNA self-organization into condensates controls mRNA cytosolic stoichiometries depen...
ORGANISM(S): Caenorhabditis elegans 
2023-09-12 | GSE213222 | GEO
ProteomicsP-body proteome
LC-MS/MS was conducted to characterize the proteome of P-bodies in HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2023-10-12 | PXD034559 | Pride
Human P-body 
GenomicsPurification-sequencing of RNA super-assemblies.
Purification-sequencing of RNA super-assemblies.
PRJNA879772 | ENA
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