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GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. While previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here we develop a sensitive and scalable protocol t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-31 | MSV000093213 | MassIVE
eHAP or U87MG cells were stably transduced with lentiCas9-Blast (Addgene, #52962) and subsequently selected using Blasticidin. Then, 300 million eHAP or U87MG cells that constitutively express Cas9 were transduced with lentiGuide-Puro from the Brunello library at MOI 0.3. Cells were then selected w...
ORGANISM(S): Homo sapiens 
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