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ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the indepth analysis of human serum proteoforms
In this study, we describe a straightforward protocol for intact proteoform sample preparation based on depletion of albumin and immunoglobulins followed by simplified fractionation of remaining serum proteins via polyacrylamide gel electrophoresis. After molecular weight-based fractionation, we sup...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-02 | MSV000092574 | MassIVE
ProteomicsTop-down mass spectrometry analysis of capsid proteins of recombinant adeno-associated virus using multiple ion activations and proton transfer charge reduction
Here, we present our investigation of the viral proteins of the capsid of adeno-associated viruses via top-down mass spectrometry on the LC timescale. Characterization was improved through the use of proton transfer charge reduction
ORGANISM(S): Human Aav 
2024-06-14 | MSV000095045 | MassIVE
ProteomicsTargeted Quantification of Proteoforms in Human Samples by Proteoform Reaction Monitoring
Here, we describe an approach based on the principle of PRM for the measurement of intact proteoforms by targeted top-down proteomics termed Proteoform Reaction Monitoring (PfRM). We explore the ability of our method to circumvent traditional limitations of top-down proteomics such as sensitivity an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-21 | MSV000091774 | MassIVE
ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the in-depth analysis of human serum proteoforms
After molecular weight-based fractionation, we supplemented the traditional liquid chromatography tandem mass spectrometry (LC-MS2) data acquisition with high-field asymmetric waveform ion mobility spectrometry (FAIMS), which served as an additional separation dimension to further simplify serum pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-02-16 | MSV000091309 | MassIVE
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsUnique Histone Proteoforms by Differential Top Down MS
H3.1 and H3.3 quadrupole isolated and fragmented by ETD and EThcD on an Orbitrap Fusion Lumos. Comparison of two cell lines.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-14 | MSV000079191 | MassIVE
ProteomicsBioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
Datafiles for Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
ORGANISM(S): Equus Caballus (ncbitaxon:9796) Bos Taurus (ncbitaxon:9913) 
2021-04-13 | MSV000087182 | MassIVE
ProteomicsImproved label-free quantification of intact proteoforms using field asymmetric ion mobility spectrometry
Recent advances in Orbitrap instrumentation, the acquisition of both intact and fragmentation high-resolution mass spectra can be achieved without the need to average time-domain transients prior to Fourier transform. This allows the application of multiple FAIMS compensation voltages in the same li...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2023-04-02 | MSV000091610 | MassIVE
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