Sort   by:  
 Page size 
ProteomicsFRET monitoring of transcription factor activities in living bacteria
Abstract: Bacteria adapt to the constantly changing environments largely by transcriptional regulation through the activities of various transcription factors (TFs). However, techniques that monitor the in situ TF-promoter interactions in living bacteria are lacking. Herein, we developed a whole-cel...
ORGANISM(S): Escherichia coli Bacteria 
2022-10-13 | PXD030973 | Pride
Transcription factor  promoter  fret  fluorescent unnatural amino acid  genetic codon expansion 
ProteomicsDishevelled-3 protein conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we developed the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD013085 | Pride
Dishevelled Casein kinase 1 ɛ Conformation Phosphorylation Flash-based fret approach 
UnknownEvaluation of FRET X for single-molecule protein fingerprinting.
Not available
S-EPMC8546410 | biostudies-literature
UnknownInteractions of Nucleosomes with Acidic Patch-Binding Peptides: A Combined Structural Bioinformatics, Molecular Modeling, Fluorescence Polarization, and Single-Molecule FRET Study.
Not available
S-EPMC10606924 | biostudies-literature
UnknownMonitoring metal-amyloid-β complexation by a FRET-based probe: design, detection, and inhibitor screening.
Not available
S-EPMC6349019 | biostudies-literature
UnknownConformational dynamics of titin PEVK explored with FRET spectroscopy.
Not available
S-EPMC3471467 | biostudies-literature
UnknownTime-resolved FRET and NMR analyses reveal selective binding of peptides containing the LC3-interacting region to ATG8 family proteins.
Not available
S-EPMC6755805 | biostudies-literature
UnknownHighly Disordered Amyloid-β Monomer Probed by Single-Molecule FRET and MD Simulation.
Not available
S-EPMC5984999 | biostudies-literature
UnknownMeasuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay.
Not available
S-EPMC4692690 | biostudies-literature
ProteomicsShedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities
Proteolysis is a universal process, as proteases play a pivotal role in modulating numerous signaling pathways. By hydrolyzing peptide bonds within their target proteins, proteases control their fate and function. Understanding the temporal and spatial dynamics of proteolytic events, including the p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-04 | PXD075809 | Pride
Sort   by:  
 Page size