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Three independent biological replicates of HCT116 cells transiently transfected with FLAG-tagged RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 ...
ORGANISM(S): Homo Sapiens (human) 
HCT116 cells transiently transfected with FLAG-tagged RPS7, RPS17, or RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% Na-deoxychol...
ORGANISM(S): Homo Sapiens (human) 
Phosphorylation status of yeast 26S proteasome subunits was analyzed by phos-tag diagonal electrophoresis and GeLC-MS analysis.
ORGANISM(S): Homo Sapiens (human) 
Phosphorylation status of human 26S proteasome subunits was analyzed by phos-tag diagonal electrophoresis and GeLC-MS analysis.
ORGANISM(S): Homo Sapiens (human) 
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. We isolate...
ORGANISM(S): Mus Musculus (mouse) 
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. Htra1-KO m...
ORGANISM(S): Mus Musculus (mouse) 
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