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ProteomicsMitochondrial proteome of ES1 KO mouse brain
Mitochondrial fraction was obtained using the mitochondrial isolation kit (KC010100, BioChain Institute). Each tissue was finely chopped and homogenized 40 strokes in two volumes of mitochondrial isolation buffer, and then centrifuged at 600×g for 10 min. The supernatant was collected and centrifuge...
ORGANISM(S): Mus Musculus (mouse) 
2022-05-12 | PXD025954 | JPOST Repository
ProteomicsMitochondrial proteome of ES1 KO mouse liver
Mitochondrial fraction was obtained using the mitochondrial isolation kit (KC010100, BioChain Institute). Each tissue was finely chopped and homogenized 40 strokes in two volumes of mitochondrial isolation buffer, and then centrifuged at 600×g for 10 min. The supernatant was collected and centrifuge...
ORGANISM(S): Mus Musculus (mouse) 
2022-05-12 | PXD025963 | JPOST Repository
ProteomicsEffect of oncogenic transformation on metabolism 03
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004848 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set07
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004835 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set06
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004834 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set02
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004830 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set03
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004831 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set05
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004833 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set04
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004832 | JPOST Repository
ProteomicsTIG-3 quantification of metabolic enzymes set01
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
2016-12-03 | PXD004829 | JPOST Repository
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