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UnknownA microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity.
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S-EPMC10301948 | biostudies-literature
TranscriptomicsAgo2-bound short RNAs in HeLaS3 cell line
Characterization of AGO2 bound short RNAs from nuclear extracts of HeLaS3 cell line. Nuclei were isolated from HeLaS3 cells; a fraction of Nuclear lysates was harvsted and sequenced as the 'input' samples. The rest of nuclear lysates were Immunoprecipitated using AGO2 monoclonal antibodies or Isotyp...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2084 | biostudies-arrayexpress
TranscriptomicsAgo1-bound short RNAs in HeLaS3 cell line
Characterization of AGO1 bound short RNAs from nuclear extracts of HeLaS3 cell line. Nuclei were isolated from HeLaS3 cells; a fraction of Nuclear lysates was harvsted and sequenced as the input samples. The rest of nuclear lysates were immunoprecipitated using AGO1 monoclonal antibodies or Isotype ...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2129 | biostudies-arrayexpress
TranscriptomicsAGO2 bound short RNAs in Jurkat cell line
Characterization of AGO2 bound short RNAs from nuclear extracts of Jurkat cell line. Nuclei were isolated from cells; a fraction of Nuclear lysates was harvested and sequenced as the input samples. The rest of nuclear lysates were Immunoprecipitated using AGO2 monoclonal antibodies or Isotype matche...
ORGANISM(S): Homo sapiens 
2015-05-17 | E-MTAB-2104 | biostudies-arrayexpress
TranscriptomicsCharacterization of microRNA Expression Profiles in human primary differentiated T lymphocytes
Activation of the T cell-mediated immune response has been associated with changes in the expression of specific miRNAs. However, the role of miRNAs in the development of an effective immune response is just beginning to be explored. Here we report a microRNA expression profile showing a list of mic...
ORGANISM(S): Homo sapiens 
2009-12-30 | E-GEOD-16453 | biostudies-arrayexpress
TranscriptomicsMiR-21 function in T-lymphocytes
T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral trans...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37213 | biostudies-arrayexpress
TranscriptomicsIdentification of miR-21 targets in Jurkat cells by AGO2 immunoprecipitation
In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarr...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37212 | biostudies-arrayexpress
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