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TranscriptomicsToxic gain of function from mutant FUS protein is crucial to trigger cell autonomous motor neuron loss
FUS is an RNA-binding protein involved in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Cytoplasmic FUS-containing aggregates are often associated with concomitant loss of nuclear FUS. Whether loss of nuclear FUS function, gain of a cytoplasmic function, or a combination of ...
ORGANISM(S): Mus musculus 
2016-03-14 | E-GEOD-78730 | biostudies-arrayexpress
TranscriptomicsFUS KO mRNA sequencing and anti-FUS RNA immunoprecipitation sequencing
The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced. RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by...
ORGANISM(S): Homo sapiens 
2016-06-13 | E-GEOD-71812 | biostudies-arrayexpress
TranscriptomicsIdentification of FUS RNA targets in HeLa cells
We identified a landscape of FUS-binding RNA targets in HeLa cells. The majority of the FUS binding sites are in introns of pre-mRNAs and less are in exons and untranslated regions. Significant FUS binding in introns flanking cassette exons, long intron (>100kb) containing transcripts and noncoding...
ORGANISM(S): Homo sapiens 
2013-12-16 | E-GEOD-50178 | biostudies-arrayexpress
TranscriptomicsIdentification of in vivo, conserved, FUS RNA binding sites and the impact of FUS on the neuronal transcriptome
FUS, an RNA binding protein was recently implicated in Amyotrophic Lateral Sclerosis (ALS). ALS is a fatal neurodegenerative disease. We report the identification of the conserved neuronal RNA targets of FUS and the assessment of the impact of FUS depletion on the neuronal transcriptome. We identif...
ORGANISM(S): Homo sapiens 
2013-01-25 | E-GEOD-43308 | biostudies-arrayexpress
TranscriptomicsExpression profiles of primary cortical neurons after knocking down Fus
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary cortical neurons by using exon-sensitive microarray technology. We analyzed total RNA of primary cortical neuron infected with lentivirus expressing shRNA against mouse ...
ORGANISM(S): Mus musculus 
2012-07-23 | E-GEOD-36153 | biostudies-arrayexpress
GenomicsHITS-CLIP analysis of FUS in lineage-committed cells.
In the present study, we performed HITS-CLIP analysis for FUS using mouse brain to extensively characterize tits RNA-binding sites and functional roles in RNA metabolisms. We identified preferential binding of FUS to stem-and-loop structures but without any discernible consensus motifs. FUS was pref...
ORGANISM(S): Mus musculus 
2012-07-26 | E-GEOD-37190 | biostudies-arrayexpress
ProteomicsCharacterization of genetic loss-of-function of Fus in zebrafish
The RNA-binding protein FUS is implicated in transcription, alternative splicing of neuronal genes and DNA repair. Mutations in FUS have been linked to human neurodegenerative diseases such as ALS (amyotrophic lateral sclerosis). We genetically disrupted fus in zebrafish (Danio rerio) using the CRIS...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2018-06-15 | PXD004876 | Pride
Crispr Zebrafish Fus protein 
TranscriptomicsExpression signatures of human HeLa cells with RNAi targetting A1 hnRNP, FUS, H hnRNP and p68-DDX5
Transcription profiling of human HeLa cells (cervical cancer cell line) transfected with a plasmid expressing shRNAs cloned into the pSuper expression vector compared to emprty vector negative controls for transfection. Four different RNA interference treatments targetted: A1 hnRNP (HNRNPA1, Heterog...
ORGANISM(S): Homo sapiens 
2008-08-20 | E-GEOD-12058 | biostudies-arrayexpress
ProteomicsIFNγ protects motor neurons from oxidative stress via enhanced global protein synthesis in FUS-associated Amyotrophic Lateral Sclerosis
Amyotrophic lateral sclerosis type 6 (ALS6) is a familial subtype of ALS linked to Fused in Sarcoma (FUS) gene mutation. FUS mutations lead to decreased global protein synthesis, but the mechanism that drives this has not been established. Here, we used ALS6 patient-derived induced pluripotent stem ...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD038042 | Pride
Als  als6  fus  protein synthesis  ifny 
GenomicsThe oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia
Fusion proteins involving the ETS factor ERG have been associated with multiple cancers such as Ewing's sarcoma and prostate cancer. In acute myeloid leukemias harboring t(16;21) another ERG fusion protein is expressed, FUS-ERG. Here, we found that this FUS-ERG oncofusion protein acts in the contex...
ORGANISM(S): Homo sapiens 
2015-06-23 | E-GEOD-60477 | biostudies-arrayexpress
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