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TranscriptomicsSmall non-coding RNA expression in murine germ cells
In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in...
ORGANISM(S): Mus musculus 
2011-06-10 | E-GEOD-24822 | biostudies-arrayexpress
ProteomicsAPEX2-MCM2 proximity labeling with high-resolution quantitative mass spectrometry in ESC
Through the APEX2 technique, we identified MCM2-interacting proteins associated with the replication initiation complex. After excluding proteins from the negative controls, we detected a substantial number of interacting proteins in the positive groups of both cell types. This research establishes ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD054939 | Pride
Apex2  mcm2  dna replication  proximity labeling 
TranscriptomicsThe histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression
Recent studies have identified a Lys 27-to-methionine (K27M) mutation at one allele of H3F3A, one of the two genes encoding histone H3 variant H3.3, in 60% of high-grade pediatric glioma cases. The median survival of this group of patients after diagnosis is ∼1 yr. Here we show that the levels of H3...
ORGANISM(S): Homo sapiens 
2014-09-20 | E-GEOD-61586 | biostudies-arrayexpress
TranscriptomicsEpigenetic dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis
Studies on the epigenetic regulation of gene expression during germ cell development are still at the beginning stage. In the present study, we used our highly specific 5hmC-labeling and enrichment technique coupled with DNA deep-sequencing to profile the global 5hmC distribution in 8 serial stages ...
ORGANISM(S): Mus musculus 
2012-03-12 | E-GEOD-35005 | biostudies-arrayexpress
GenomicsPharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma [ChIP-seq]
ChIP-Seq analysis of a pediatric human diffuse intrinsic pontine glioma (DIPG) cell line SF8628, harboring the K27M mutation. Goal was to obtain quantitative estimates of K27me3 immunoprecipitation change between vehicle-treated SF8628 cells [dimethyl sulfoxide, (DMSO)] and SF8628 cells incubated wi...
ORGANISM(S): Homo sapiens 
2014-07-20 | E-GEOD-59496 | biostudies-arrayexpress
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