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ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
ProteomicsCoupling SDS-PAGE to capillary zone electrophoresis-tandem mass spectrometry for high-resolution top-down proteomics analysis of intact histone proteoforms
Top-down mass spectrometry (TDMS) is a powerful tool to reveal the mechanism of epigenetic regulation by histones. However, the high similarity of histone variants and their highly dynamic post-translational modifications (PTMs) make them as on-going challenge analytes for separation with traditiona...
ORGANISM(S): Bos taurus (Bovine) 
2024-10-15 | PXD048061 | Pride
Sds-page Top-down proteomics Post-translational modifications Histone proteoform Cze-ms/ms 
ProteomicsFinding the optimal degassing condition for linear polyacrylamide coated capillaries used in capillary zone electrophoresis-mass spectrometry-based top-down proteomics
LPA-coated capillaries are widely used; however, the quality of the coating—which directly affects separation performance and analyte identification—can vary significantly depending on the individual performing the procedure. A critical factor in achieving consistent coating quality is the degassing...
ORGANISM(S): Homo sapiens (Human) 
2026-05-14 | PXD067278 | Pride
Degass Polyacrylamide-coated capillary Top-down proteomics Reproducibility Capillary zone electrophoresis 
ProteomicsHigh-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry
A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hun...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-08-04 | PXD063043 | Pride
Nanoparticle protein corona Bottom-up proteomics High-throughput Cze-ms/ms Biomarker 
ProteomicsThe TET-Sall4-BMP regulatory axis controls craniofacial cartilage development
Craniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis of CFM pathogenesis is largely unknown. Here, we employ the zebrafish model to investigate mechanisms of CFM pathogenesis. In early embryos...
ORGANISM(S): Danio Rerio 
2024-02-02 | PXD049147 |
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