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ProteomicsAn improved nanoflow RPLC-CZE-MS/MS system with high peak capacity and sensitivity for nanogram bottom-up proteomics
Novel mass spectrometry (MS)-based proteomic tools with extremely high sensitivity and high peak capacity are required for comprehensive characterization of protein molecules in mass-limited proteome samples. We previously reported a nanoflow RPLC-CZE-MS/MS (nanoRPLC-CZE-MS/MS) system for deep botto...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014392 | Pride
Human cells Nanorplc-cze-ms/ms Bottom-up proteomics Nanograms 
UnknownImproved Nanoflow RPLC-CZE-MS/MS System with High Peak Capacity and Sensitivity for Nanogram Bottom-up Proteomics.
Not available
S-EPMC7545725 | biostudies-literature
ProteomicsPhosphoproteomics using CZE-MS/MS
Phosphoproteomics requires better separation of phosphopeptides to boost the coverage of the phosphoproteome. We argue that an alternative separation method that produces orthogonal phosphopeptide separation to the widely used LC needs to be considered. Capillary zone electrophoresis (CZE) is one im...
ORGANISM(S): Homo sapiens (Human) 
2019-01-16 | PXD012255 | Pride
Cze-ms/ms Hct116 cell line Phosphoproteomics 
ProteomicsIntegrated MICROFASP method with CZE based fractionation technique and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample
We report a loss-less two dimensional (2D) separation platform that integrated capillary zone electrophoresis (CZE) fractionation and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample. Protein digest was injected into the linear polyacrylamide coated capillary followed ...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD052735 | Pride
Submicrogram sample Two dimensional separation Capillary zone electrophoresis Microfasp method Proteome profiling 
ProteomicsLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain
Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new proteoform biomarkers of diseases. There are few published spatially re...
ORGANISM(S): zebrafish metagenome 
2023-03-11 | PXD027741 | Pride
Spatially resolved top-down proteomics  capillary zone electrophoresis-tandem mass spectrometry  laser capture microdissection  zebrafish brain  proteoform  high sensitivity  neuropeptide hormone activity  motor activity 
ProteomicsCZE-MS/MS-based quantitative top-down proteomics reveals significant proteoform differences between healthy and Alzheimer's disease human brains
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive decline and pathological protein aggregation. Comprehensive protein characterization of human brains of AD patients and comparison with healthy samples is critical for pursuing a better understanding of the molecular...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD065459 | Pride
Top-down proteomics Human brain Proteoform Tau phosphorylation. Cze-ms/ms Alzheimer's disease 
UnknownIn-line sample trap columns with diatomite for large-volume injection in CZE-IM-MS.
Not available
S-EPMC10099630 | biostudies-literature
UnknownLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain.
Not available
S-EPMC9066772 | biostudies-literature
ProteomicsMicro-scale RPLC-CZE-MS/MS for deep and highly sensitive bottom-up proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. Howe...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD009991 | Pride
Micro-scale rplc Cze-ms/ms 
UnknownRapid identification of antibody impurities in size-based electrophoresis via CZE-MS generated spectral library.
Not available
S-EPMC11364755 | biostudies-literature
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