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ProteomicsIntegrated MICROFASP method with CZE based fractionation technique and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample
We report a loss-less two dimensional (2D) separation platform that integrated capillary zone electrophoresis (CZE) fractionation and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample. Protein digest was injected into the linear polyacrylamide coated capillary followed ...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD052735 | Pride
Submicrogram sample Two dimensional separation Capillary zone electrophoresis Microfasp method Proteome profiling 
ProteomicsAn improved nanoflow RPLC-CZE-MS/MS system with high peak capacity and sensitivity for nanogram bottom-up proteomics
Novel mass spectrometry (MS)-based proteomic tools with extremely high sensitivity and high peak capacity are required for comprehensive characterization of protein molecules in mass-limited proteome samples. We previously reported a nanoflow RPLC-CZE-MS/MS (nanoRPLC-CZE-MS/MS) system for deep botto...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014392 | Pride
Human cells Nanorplc-cze-ms/ms Bottom-up proteomics Nanograms 
UnknownImproved Nanoflow RPLC-CZE-MS/MS System with High Peak Capacity and Sensitivity for Nanogram Bottom-up Proteomics.
Not available
S-EPMC7545725 | biostudies-literature
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