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ProteomicsQualitative and quantitative top-down proteomics of human colorectal cancer cell lines identified 23000 proteoforms and revealed drastic proteoform-level differences between metastatic and non-metastatic cancer cells
Understanding cancer metastasis at the proteoform level is crucial for discovering new protein biomarkers for cancer diagnosis and drug development. Proteins are the primary effectors of function in biology and proteoforms from the same gene can have drastically different biological functions. Here,...
ORGANISM(S): Homo sapiens (Human) 
2023-01-05 | PXD029703 | Pride
Top-down proteomics Proteoform Cancer metastasis Colorectal cancer 
ProteomicsProteoform Imaging by Combining MALDI MSI and High-sensitivity Top-down Proteomics
Spatially resolved characterization of proteoforms has great potential to significantly advance our understanding of physiological and disease mechanisms. However, it faces challenges regarding throughput and coverage. Herein, we developed a robust method for high-throughput proteoform imaging (HTPi...
ORGANISM(S): Mus Musculus 
2024-12-03 | PXD058552 |
ProteomicsInfluence of in-source ion activation energies on artificial proteoform truncation
In order to elucidate the influence of in-source ion activation on artificial proteoform truncation, intact proteoforms from Escherichia coli lysate (enriched by proteoforms smaller than 20 kDa) were analyzed using LC-FAIMS-MS/MS with varying in-source ion activation energies.
ORGANISM(S): Escherichia coli 
2025-11-11 | PXD066719 | Pride
E. coli Proteoform Top-down-proteomics In-source fragmentation 
ProteomicsCZE-MS/MS-based quantitative top-down proteomics reveals significant proteoform differences between healthy and Alzheimer's disease human brains
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive decline and pathological protein aggregation. Comprehensive protein characterization of human brains of AD patients and comparison with healthy samples is critical for pursuing a better understanding of the molecular...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD065459 | Pride
Top-down proteomics Human brain Proteoform Tau phosphorylation. Cze-ms/ms Alzheimer's disease 
UnknownProteoform Suite: Software for Constructing, Quantifying, and Visualizing Proteoform Families.
Not available
S-EPMC5770237 | biostudies-literature
ProteomicsSpatially-Resolved Top-Down Proteomics of Zebrafish Brain Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry
Understanding region-specific proteoform profiles in the brain is crucial for deciphering neural function and identifying potential therapeutic targets. Here, we present a comprehensive label-free quantitative top-down proteomics (TDP) analysis of distinct zebrafish brain regions using capillary zon...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD070925 | Pride
Proteoform Top-down proteomics Zebrafish brainregions 
UnknownExpanding Proteoform Identifications in Top-Down Proteomic Analyses by Constructing Proteoform Families.
Not available
S-EPMC5807004 | biostudies-literature
UnknownElucidating Proteoform Families from Proteoform Intact-Mass and Lysine-Count Measurements.
Not available
S-EPMC4917391 | biostudies-literature
TranscriptomicsPositional proteomics reveals differences in N-terminal proteoform stability
To understand the impact of alternative translation initiation on a proteome, we performed the first study on protein turnover using positional proteomics and ribosome profiling to distinguish between N-terminal proteoforms of individual genes. Overall, we monitored the stability of 1,941 human N-te...
ORGANISM(S): Homo sapiens 
2015-10-23 | E-GEOD-74279 | biostudies-arrayexpress
TranscriptomicsPositional proteomics reveals differences in N-terminal proteoform stability
To understand the impact of alternative translation initiation on a proteome, we performed the first study on protein turnover using positional proteomics and ribosome profiling to distinguish between N-terminal proteoforms of individual genes. Overall, we monitored the stability of 1,941 human N-te...
ORGANISM(S): Homo sapiens 
2015-10-23 | GSE74279 | GEO
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