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Proteomics,Multiomicsembryo_proteome
We collected over 8000 mouse embryos of each developmental stage (zygote, 2-cell, 4-cell, 8-cell, morula, blastocyst) and performed tandem mass tag (TMT)-based quantitative mass spectrometry and identified nearly 5000 proteins for each stage. In-depth analysis indicates that protein expression profi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2015-12-10 | MSV000079417 | MassIVE
TranscriptomicsInducible iPS Cells Support Full-term Development of Tetraploid Blastocyst–Complemented Embryos
Differentiated somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by forced expression of four transcription factors—Oct4, Sox2, Klf4, and c-Myc. However, it remains undetermined whether the reprogrammed iPS cells are fully pluripotent, resembling normal embryonic stem (ES...
ORGANISM(S): Mus musculus 
2010-08-18 | E-GEOD-17004 | biostudies-arrayexpress
TranscriptomicsGene expression in pluripotent and TSKM-iPS induction cells.
We found that Tet1 (T) can substitute Oct4 and initiates somatic cell reprogramming in combination with Sox2 (S), Klf4 (K) and c-Myc (M). Moreover, the TSKM secondary reprogramming can proceed rapidly with widespread accompanying increase of 5hmC and 5mC at TSS and ES-active regulation regions follo...
ORGANISM(S): Mus musculus 
2013-03-18 | E-GEOD-39637 | biostudies-arrayexpress
TranscriptomicsSingle Cell Sequencing Identifies Key Epigenetic Regulators in Nuclear Transfer Mediated Reprogramming.
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2016-05-16 | E-GEOD-70608 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of sequentially reprogrammed iPS cells reveals key epigenetic modifications correlated with reduced pluripotency of iPS cells [sRNA-seq]
Full pluripotency of induced pluripotent stem (iPS) cells has been determined as viable all-iPS mice can be generated through tetraploid complementation. Subsequently, activation of imprinted Dlk-Dio3 gene cluster has been suggested to correlate with the pluripotency of iPS cells1. However, evidence...
ORGANISM(S): Mus musculus 
2013-12-06 | E-GEOD-36291 | biostudies-arrayexpress
GenomicsGenome-wide maps of 5hmC/5mC state in pluripotent and (T-)iPS induction cells.
We performed 5hmC/5mC DNA Immunoprecipitation followed high-throughput sequencing using the cell sample along the whole TSKM secondary reprogramming system. The TSKM 0D is the fibroblasts deried from TSKM-iPS mouse as the starting cells of the reprogramming.The intermediate cells is 3-days induced c...
ORGANISM(S): Mus musculus 
2013-03-18 | E-GEOD-39638 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of sequentially reprogrammed iPS cells reveals key epigenetic modifications correlated with reduced pluripotency of iPS cells
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2013-12-06 | E-GEOD-36294 | biostudies-arrayexpress
GenomicsGenome-wide maps of 5hmC state in pluripotent and (T-)iPS induction cells.
We performed 5hmC DNA Immunoprecipitation followed high-throughput sequencing using R1 ESCs, OT ESCs, TSKM ESCs, Day3 TSKM and OSKM induction cells. We compared the profiling of 5-hydroxymethylcytosine modifications in these different cell lines. We found that: the TSKM and OT iPSCs shared similar 5...
ORGANISM(S): Mus musculus 
2012-11-17 | E-GEOD-33781 | biostudies-arrayexpress
GenomicsSingle Cell Sequencing Identifies Key Epigenetic Regulators in Nuclear Transfer Mediated Reprogramming [RRBS]
Differentiated cell can be reprogrammed into totipotent embryo through somatic cell nuclear transfer (SCNT). However, this process is highly inefficient and most cloned embryos arrest at certain developmental stages. Through single cell sequencing combined with embryo biopsy, here we generate a glob...
ORGANISM(S): Mus musculus 
2016-05-17 | E-GEOD-70607 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of sequentially reprogrammed iPS cells reveals key epigenetic modifications correlated with reduced pluripotency of iPS cells [RNA-seq]
Full pluripotency of induced pluripotent stem (iPS) cells has been determined as viable all-iPS mice can be generated through tetraploid complementation. Subsequently, activation of imprinted Dlk-Dio3 gene cluster has been suggested to correlate with the pluripotency of iPS cells1. However, evidence...
ORGANISM(S): Mus musculus 
2013-12-06 | E-GEOD-36290 | biostudies-arrayexpress
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