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TranscriptomicsTranscription profiling of mouse FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells
Identification of genes regulated by GATA-1 independent of the cofactor FOG-1. Experiment Overall Design: A conditionally activated FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells. Transcipt levels were compared in cells untreated or treated with...
ORGANISM(S): Mus musculus 
2008-06-15 | E-GEOD-6958 | biostudies-arrayexpress
GenomicsGenome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy
We report ChIP-Seq data for GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential. We introduced HA-tagged GATA-1 or V205G into G1ME cells via retroviral transduction. The cells were crossli...
ORGANISM(S): Mus musculus 
2012-07-30 | E-GEOD-35644 | biostudies-arrayexpress
TranscriptomicsGATA-1 and heme regulate the erythroid cell transcriptome.
Alas2 gene encodes the rate-limiting enzyme in heme biosynthesis. CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing ...
ORGANISM(S): Mus musculus 
2016-01-07 | E-GEOD-74371 | biostudies-arrayexpress
TranscriptomicsCofactor-mediated Restriction of GATA-1 Chromatin Occupancy Coordinates Lineage-specific Gene Expression
This SuperSeries is composed of the following subset Series: GSE35644: Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy GSE35695: Differential gene regulation by disease-associated mutants of GATA-1 during megakaryocyte differentiation Refer to individual Series
ORGANISM(S): Mus musculus 
2012-07-29 | E-GEOD-35708 | biostudies-arrayexpress
TranscriptomicsGATA-1-regulated erythroid microRNAs
We employed a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. To search for GATA-1-regulated erythroid miRNAs, we used the Gata-1– erythroblast line G1E. These cells proliferate in culture as immature ery...
ORGANISM(S): Mus musculus 
2008-02-05 | E-GEOD-10134 | biostudies-arrayexpress
GenomicsInsights into GATA-1-Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis
The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, we p...
ORGANISM(S): Mus musculus 
2009-11-25 | E-GEOD-16594 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of G1E cells which are Gata-1 erythroid-committed cell line derived from targeted disruption of Gata-1 in embryonic stem cells to study the global regulation of erythroid gene expression by transcription factor GATA-1
G1E cells are a Gata-1 erythroid-committed cell line derived from targeted disruption of Gata-1 in embryonic stem cells. The ER4 subclone contains an inducible form of Gata-1 (Gata-1-ER, Gata-1 fused to the estradiol receptor ligand binding domain). We performed transcriptome analysis using this cel...
ORGANISM(S): Mus musculus 
2007-12-15 | E-GEOD-628 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of murine GATA-1 wild type and mutant fetal megakaryocytes to understand the molecular basis for the megakaryocytic hyperproliferation phenotype of the GATA-1deltaN mutants
Background: Children with Down syndrome (DS) are predisposed to acute megakaryoblastic leukemia (AMKL, or AML M7) and a related myeloid disorder, referred to as transient myeloproliferative disorder (TMD), or transient leukemia (TL). Recently, acquired mutations in the erythroid/megakaryocytic linea...
ORGANISM(S): Mus musculus 
2007-08-31 | E-GEOD-2433 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of megakaryocyte cells from Gata-1 knock out mice and wild type mice to study the differential requirements for the activation domain and FOG-interaction surface of GATA-1 in megakaryocyte gene expression and development
Hematopoietic progenitor cells were isolated from 13.5 day mouse fetal livers by lineage depletion and expanded for three days. Fetal livers were isolated from both wild type and Gata-1 knock embryos. Gata-1 knock embryos contain a deletion of the Gata-1 promoter sequence that results in undetectabl...
ORGANISM(S): Mus musculus 
2007-09-04 | E-GEOD-2527 | biostudies-arrayexpress
TranscriptomicsFoxo3 Regulated Genes in G1E-ER-GATA-1 Cells
The ensemble of Foxo3-regulated genes in the erythroid G1E-ER-GATA-1 cell line was determined by knocking down Foxo3 using siRNA, and measuring genome wide transcription by microarray analysis G1E-ER-GATA-1 cells were treated with control or Foxo3-specific siRNA by nucleofection at t = 0 h and t = ...
ORGANISM(S): Mus musculus 
2014-08-09 | E-GEOD-60137 | biostudies-arrayexpress
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