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TranscriptomicsTranscription profiling of S. cervisiae wild type (BY4741) and Puf3 mutant cells grown in minimal medium supplemented with 3% glycerol
Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mi...
ORGANISM(S): Saccharomyces cerevisiae 
2005-11-01 | E-SMDB-2623 | biostudies-arrayexpress
TranscriptomicsWild-type versus trf4, trf5, and trf4-DADA mutant cells
Measurement of the relative changes of gene expression of S. cerevisiae cells lacking either trf4 (trf4delta) or trf5 (trf5delta), and trf4delta/TRF4-DADA mutants compared to wild-type (WT) cells using yeast oligo microarrays that contain features representing all annotated yeast ORFs, ncRNAs, intro...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-16103 | biostudies-arrayexpress
MetabolomicsPseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis Mediator mutants

Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediato...

2024-02-19 | MTBLS8105 | MetaboLights
ProteomicsPom1-AS quantitative phosphoproteomics
Complex phosphorylation-dependent signaling networks underlie the coordination of cellular growth and division. In the fission yeast S. pombe, the DYRK family protein kinase regulates cell cycle progression through the mitotic inducer Cdr2, and controls cell polarity through unknown targets. Here, w...
ORGANISM(S): Schizosaccharomyces pombe 
2015-03-09 | PXD001649 | Pride
Pom1 Silac Pombe Phosphoproteomics 
TranscriptomicsShg1p associated RNAs
RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-15247 | biostudies-arrayexpress
TranscriptomicsPuf3delta vs. wild type comparison
Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mi...
ORGANISM(S): Saccharomyces cerevisiae 
2006-01-03 | E-GEOD-3969 | biostudies-arrayexpress
UnknownStructural insights into the stimulation of S. pombe Dnmt2 catalytic efficiency by the tRNA nucleoside queuosine.
Not available
S-EPMC5995894 | biostudies-literature
TranscriptomicsRBP40_arrays
In this study, we systematically identified the RNAs associated with a selective sample of 40 of ~600 yeast RNA-binding proteins (RBPs). To identify RNAs associated with each putative RBP, C-terminal tandem affinity purification (TAP)-tagged proteins, expressed under control of their native promoter...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-25 | E-GEOD-13135 | biostudies-arrayexpress
TranscriptomicsPuf proteins, RNA co-immunopurification
RNA-coimmunopurifications with TAP-tagged Puf proteins from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control. Cells were grown to midlog phase and harvested by centrifugation. TAP-tagged Puf proteins were affinity purified from cell-free extracts with IgG sepharose and eluted ...
ORGANISM(S): Saccharomyces cerevisiae 
2006-01-03 | E-GEOD-3968 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation with TAP-tagged Puf proteins from Saccharomyces cereviseae
RNA-coimmunopurifications with TAP-tagged Puf proteins from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control. Cells were grown to midlog phase and harvested by centrifugation. TAP-tagged Puf proteins were affinity purified from cell-free extracts with IgG sepharose and eluted ...
ORGANISM(S): Saccharomyces cerevisiae 
2005-11-01 | E-SMDB-2622 | biostudies-arrayexpress
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