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TranscriptomicsTranscription profiling of mouse single cells from primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)
Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative-PCR analysis, we defined the genome-wide transcription dynamics that create primordial germ cells (PGCs) from the epiblast, a process that exclusively segrega...
ORGANISM(S): Mus musculus 
2008-10-25 | E-GEOD-11128 | biostudies-arrayexpress
TranscriptomicsSOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state. However, without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. Here, we demonstrate specification of hPGC-like cells (hPGCLCs) from germline competent pluripo...
ORGANISM(S): Homo sapiens 
2014-12-26 | E-GEOD-60138 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp2]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-46854 | biostudies-arrayexpress
TranscriptomicsMetastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation
Specification to primordial germ cells (PGCs) occurs under the mesoderm induction signals during gastrulation. Here, we found that Akt activation in embryonic stem (ES) cells generated self-renewing spheres during mesodermal differentiation induction and that the differentiation status of the spher...
ORGANISM(S): Mus musculus 
2010-04-30 | E-GEOD-18813 | biostudies-arrayexpress
TranscriptomicsReplication Timing Reveal An Epigenetic Commitment To Differentiation Prior To Germ Layer Specification (WG_CGH, RT)
Differentiation of mouse embryonic stem cells (mESCs) is accompanied by global changes in replication timing. To elucidate this reorganization process and explore its potential impact on mouse development, we constructed genome-wide replication-timing profiles of 15 independent mouse cell types repr...
ORGANISM(S): Mus musculus 
2010-05-19 | E-GEOD-17983 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp1]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-43775 | biostudies-arrayexpress
TranscriptomicsGene-expression profile of human embryonic germ cells
Gene-expression profile of human embryonic germ cells (EGCs), primordial germ cells (PGCs), embryonal carcinoma cells (ECCs), pluripotent embryonic stem cells (ESCs) and induced pluripotent stem cells (IPSCs)
ORGANISM(S): Homo sapiens 
2018-06-28 | E-MTAB-6851 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by high throughput sequencing of primordial germ cells from e6.5, e7.5, e8.5 embryos and e7.5 somatic neighbours and Blimp1-KO mice
Primordial germ cell mRNA profiles from cells microdissected from e6.5, e7.5 and e8.5 embryos, e7.5 somatic neighbours and Blimp1-KO mice were generated by single cell library construction and sequencing in duplicate using Applied Biosystems SOLiD sequencer. Single cell library construction is descr...
ORGANISM(S): Mus musculus 
2013-07-01 | E-MTAB-1178 | biostudies-arrayexpress
ProteomicsTdrd6a regulates the aggregation of Buc into functional subcellular compartments that drive germ cell specification
In recent years, it has become clear that membrane-less structures formed through phase separation represent an important class of subcellular compartmentalization. However, little is known about how the formation or disassembly of such compartments is regulated. The Balbiani body (Bb) is a phase-se...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2018-06-13 | PXD008322 | Pride
Lc-ms/ms Immunoprecipitation Peptide pulldown 
UnknownNodal induces sequential restriction of germ cell factors during primordial germ cell specification.
Not available
S-EPMC5825842 | biostudies-literature
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