After fertilization, a plant's life relies on progression through embryogenesis and maintenance of the stem cell niches from which all post-embryonic organs arise. BABY BOOM (BBM) and other members of the AINTEGUMENTA-LIKE (AIL)/PLETHORA (PLT) clade of the AP2-type transcription factor family play i...
To reveal subclass I SnRK2-interacting proteins, we conducted co-immunoprecipitation using anti-GFP antibody from subclass I SnRK2-GFP expressing plants. Immunoprecipitates were identified by LC-MS/MS analyses.
Shieldin complex promotes DNA end joining and counters homologous recombination in BRCA1-null cells: Immunoprecipitation-mass spectrometric analysis of protein interactors of GFP-C20orf196 and GFP-FAM35A
Approximately 1 x 10^8 GFP-HA-KERP2 expressing Entamoeba histolytica cells were cross-linked in 0.5% formaldehyde (in PBS) for 10 minutes at room temperature. Cross-linking was quenched with 125 mM glycine for 5 minutes. Cells were washed once with PBS and subjected to nuclear fractionation using nu...
mTRAN proteins are components of the plant mitochondrial small subunits, thought to bind the mRNA 5' regions to initiate translation. This experiment was designed to identify the mTRAN1 binding regions on the plant mitochondrial mRNAs
LYS142, LYS143, LYS14 and LYS144 compose a family of recently duplicated transcription regulators in C. albicans. They have differentiated from one another by acquiring a largely separate set of target genes. Chromatin immunoprecipitation (ChIP) of GFP-LYS142, GFP-LYS143, GFP-LYS14 and GFP-LYS144 fo...
We established a neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high throughput sequencing (AGO2-RIP-seq) to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this technique, we identified more than two thousand miRNA target genes in hippocampal neurons, r...
A genomic overview of in vivo binding of a transcription factor FoxA-a in the ascidian 64-cell stage embryo. Fertilized eggs were electroporated with a construct encoding GFP-tagged FoxA-a. After fixation, Chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragme...
