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ProteomicsDenaturing mass photometry for rapid optimization of chemical protein-protein cross-linking reactions
Chemical cross-linking reactions (XL) are an important strategy for studying protein-protein interactions (PPIs), including low abundant sub-complexes, in structural biology. However, choosing XL reagents and conditions is laborious and mostly limited to analysis of protein assemblies that can be re...
ORGANISM(S): Escherichia coli 
2024-03-15 | PXD042549 | Pride
Mass photometry Cross-linking mass spectrometry 
ProteomicsFAIMS-GPF XL-MS: crosslinking-mass spectrometry based on gas-phase fractionation
Protein-protein interactions (PPIs) underpin nearly all cellular processes; therefore, mapping PPI and protein-protein association networks is critical for understanding how biological systems function in health and disease. However, many functionally relevant PPIs are transient and mediated by weak...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD064228 | Pride
Faims Cross-linking mass spectrometry Structural biology Interactomics 
ProteomicsA comprehensive overview to understand the dynamic R2SP complex.
Here, we used molecular biological, biochemical, biophysical and structural approaches to describe the human R2SP complex, that reveal similarities as well as significant differences compared to canonical R2TP and hints towards its specific mode of assembly and action. Cross-linking mass spectrometr...
ORGANISM(S): Escherichia coli 
2026-02-13 | PXD056494 | Pride
Proteomics Cross-linking mass spectrometry Structural biology 
ProteomicsNucleotide independant as well as ATP-hydrolysis driven steps of Rea1 Linker remodelling drive the removal of assembly factors from pre-ribosomal particles: PhoX cross-linking of Rea1 in presence of ATPgS, AMP-PNP or Apo state.
The ribosome maturation factor Rea1 (or Midasin) catalyses the removal of assembly factors from large ribosomal subunit precursors and to promotes their export from the nucleus to the cytosol. Rea1 consists of nearly 5000 amino-acid residues and belongs to the AAA+ protein family. It consists of a r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-29 | PXD053636 | Pride
Cross-linking mass spectrometry Structural biology Structural proteomics 
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