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GenomicsGlucocorticoid Receptor and Androgen Receptor DNA binding in LREX'
LREX' are a LnCAP/AR subline with natural expresison of the glucocorticoid receptor. We used the model to compare the AR and GR cistromes in prostate tissue. We determined the GR cistrone in the presence of Dex (100nM) treatment and the AR cistrome in the presene of DHT (1nM) treatment.
ORGANISM(S): Homo sapiens 
2013-12-27 | E-GEOD-51497 | biostudies-arrayexpress
TranscriptomicsRole of Serine 134 phosphorylation of the glucocorticoid receptor (GR) in glucocorticoid signaling
In response to environmental stressors and a variety of inflammatory cytokines, p38 MAPKs become directly activated. Here we report the human glucocorticoid receptor (GR) Serine 134 as a novel target for p38 MAPK. Unlike most other phosphorylation events that occur on the GR, phosphorylation of Ser1...
ORGANISM(S): Homo sapiens 
2011-10-04 | E-GEOD-28912 | biostudies-arrayexpress
GenomicsGlucocorticoid receptor profiling in U2OS cells expressing GRalpha or GRgamma isoform
To determine whether insertion of a single amino acid had an effect on Glucocorticoid receptor (GR) occupancy, we performed ChIP-seq of GRalpha and GRgamma in the same U2OS osteosarcoma cell lines, upon glucocorticoid treatment. These assays are also part of submission E-MTAB-2955
ORGANISM(S): Homo sapiens 
2014-08-08 | E-MTAB-2731 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation and transcription of mouse liver in the presence or absence of exogenous glucocorticoid to identify direct targets of the glucocorticoid receptor
The purpose of this study was to identify direct targets of the glucocorticoid receptor (GR) using an orthogonal analysis. An expression study of mouse livers in the presence or absence of exogenous glucocorticoid complemented a genome-wide location analysis on chromatin from the same livers. Thes...
ORGANISM(S): Mus musculus 
2007-09-14 | E-CBIL-38 | biostudies-arrayexpress
GenomicsLigand-dependent genomic function of glucocorticoid receptor in triple-negative breast cancer
Glucocorticoids (GC) have been widely used as coadjuvants in the treatment of solid tumors, but GC treatment may be associated with poor pharmacotherapeutic response and/or prognosis. The genomic action of GC in these tumors is largely unknown. Here we find that dexamethasone (Dex, a synthetic GC) r...
ORGANISM(S): Homo sapiens 
2015-09-18 | E-GEOD-56022 | biostudies-arrayexpress
TranscriptomicsGlucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment
We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line and the androgen receptor (AR) in the U2OS-AR cell line. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2O...
ORGANISM(S): Homo sapiens 
2021-03-09 | E-MTAB-9616 | biostudies-arrayexpress
GenomicsGlucocorticoid receptor profiling in IMR90,K562 and U2OS (ChIP-seq)
To identify the sequences responsible for recruitment of Glucocorticoid receptor (GR) to individual loci, we performed ChIP-seq and ChIP-exo that combines chromatin immunoprecipitation with an exonuclease digestion step. We performed these experiments in three cell lines : IMR90 (ATTC:CCL-186), U2O...
ORGANISM(S): Homo sapiens 
2015-02-25 | E-MTAB-2955 | biostudies-arrayexpress
TranscriptomicsRNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)
To identify the genes regulated by the Glucocorticoid Receptor (GR), we performed RNA-seq in GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment for 24 hours.
ORGANISM(S): Homo sapiens 
2019-03-11 | E-MTAB-7745 | biostudies-arrayexpress
TranscriptomicsExpression microarray screen for genes regulated by the unliganded glucocorticoid receptor
To further characterize the role of the unliganded glucocorticoid receptor (GR) in breast cells, we used an shRNA vector directed against GR to create EPH-4 mouse mammary cell lines with depleted endogenous levels of this receptor. RNA prepared from normal (EV-50) and GR-depleted (shGR-19) cells was...
ORGANISM(S): Mus musculus 
2014-05-11 | E-GEOD-51408 | biostudies-arrayexpress
TranscriptomicsGlobal Analysis of Glucocorticoid Receptor-Associated Transcripts and Identification of a Target RNA Motif
Posttranscriptional regulation is emerging as a key factor in glucocorticoid (GC)-mediated gene regulation. We investigated the role of the human glucocorticoid receptor (GR) as an RNA-binding protein and its effect on mRNA turnover in human airway epithelial cells. Cell treatment with the potent ...
ORGANISM(S): Homo sapiens 
2011-06-24 | E-GEOD-24748 | biostudies-arrayexpress
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