Glutamatergic and gabaergic neurons

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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1. CERVO Brain Research Centre, Quebec Mental Health Institute; Québec, QC, G1E 1T2, Canada. 2. Department of Psychiatry & Neuroscience, Université Laval; Québec, QC, G1V 0A6, Canada. (1)
Department of Molecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW, Australia (1)
Dept of Neurology University Hospital RWTH Aachen Pauwelsstrasse 30 52074 Aachen Germany (1)
Faculty of Health Sciences, Department of Proteomics, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark (1)
Institut du Fer a Moulin, Inserm-Sorbonne Université UMR-S 1270, Paris, France (1)
Professor, Director of Graduate Studies Neuroscience Program Department of Cell Biology, Emory University (1)

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Distinct mechanisms of non-autonomous UPRER mediated by GABAergic, glutamatergic, and octopaminergic neurons

The capacity to deal with stress declines during the aging process, and preservation of cellular stress responses is critical to healthy aging. The unfolded protein response of the endoplasmic reticulum (UPRER) is one such conserved mechanism which is critical for the maintenance of several major fu...

ORGANISM(S): Caenorhabditis elegans

2024-06-15 | E-MTAB-14132 | biostudies-arrayexpress

Distinct mechanisms of non-autonomous UPRER mediated by GABAergic, glutamatergic, and octopaminergic neurons

PRJEB76195 | ENA

Gene expression profiles of glutamatergic neuron progenitors (Neurod6-positive cells)

ORGANISM(S): Mus musculus

2012-12-08 | E-MTAB-893 | biostudies-arrayexpress

Gene expression profiles of GABAergic neuron progenitors (GAD67-GFP-positive cells)

ORGANISM(S): Mus musculus

2012-12-31 | E-MTAB-904 | biostudies-arrayexpress

Transcription profiling of microdissected and hand sorted mouse forebrain neurons to understand the extend and nature of differential expression in various cell types.

The mammalian forebrain is a tissue of stunning complexity comprised of numerous regions each containing many distinct cell types that differ in their intrinsic and synaptic physiology, morphology and connectivity. These differences are likely conferred by differential gene expression, but the exte...

ORGANISM(S): Mus musculus

2007-08-31 | E-GEOD-2882 | biostudies-arrayexpress

Coordinated Plasticity between Barrel Cortical Glutamatergic and GABAergic Neurons during Associative Memory.

Not available

S-EPMC5192352 | biostudies-literature

Topographic Organization of Glutamatergic and GABAergic Parvalbumin-Positive Neurons in the Lateral Habenula.

Not available

S-EPMC11255393 | biostudies-literature

A Whole-Brain Connectivity Map of VTA and SNc Glutamatergic and GABAergic Neurons in Mice.

Not available

S-EPMC8733212 | biostudies-literature

Glutamatergic and gabaergic ventral BNST neurons differ in their physiological properties and responsiveness to noradrenaline.