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We comprehensively investigated the neural-splicing using P19 cells, set up nine filtering conditions, and obtained 262 candidate exons (236 genes). Results of semi-quantitative RT-PCR in randomly selected 30 candidates suggested that 87% of the candidates were actually changed more than double comp...
ORGANISM(S): Mus musculus 
Mitochondrial fraction was obtained using the mitochondrial isolation kit (KC010100, BioChain Institute). Each tissue was finely chopped and homogenized 40 strokes in two volumes of mitochondrial isolation buffer, and then centrifuged at 600×g for 10 min. The supernatant was collected and centrifuge...
ORGANISM(S): Mus Musculus (mouse) 
Mitochondrial fraction was obtained using the mitochondrial isolation kit (KC010100, BioChain Institute). Each tissue was finely chopped and homogenized 40 strokes in two volumes of mitochondrial isolation buffer, and then centrifuged at 600×g for 10 min. The supernatant was collected and centrifuge...
ORGANISM(S): Mus Musculus (mouse) 
Genomic binding of 203 transcriptional regulatory proteins under various conditions. Hybridizations of fluorescently-labelled immunoprecipitated vs. whole-cell extract samples on S. cerevisiae intergenic arrays.
ORGANISM(S): Saccharomyces cerevisiae 
To facilitate collaborative research efforts between multi-investigator teams using DNA microarrays, we identified sources of error and data variability between laboratories and across microarray platforms and methods to accommodate this variability. RNA expression data were generated in seven labo...
ORGANISM(S): Mus musculus 
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