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Chromatin immunoprecipitation genome wide location analysis of yeast transcriptional regulators under multiple growth conditions

ABSTRACT: Genomic binding of 203 transcriptional regulatory proteins under various conditions. Hybridizations of fluorescently-labelled immunoprecipitated vs. whole-cell extract samples on S. cerevisiae intergenic arrays.

INSTRUMENT(S): vortex

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Elizabeth Herbolsheimer 

PROVIDER: E-WMIT-10 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA-binding transcriptional regulators interpret the genome's regulatory code by binding to specific sequences to induce or repress gene expression. Comparative genomics has recently been used to identify potential cis-regulatory sequences within the yeast genome on the basis of phylogenetic conservation, but this information alone does not reveal if or when transcriptional regulators occupy these binding sites. We have constructed an initial map of yeast's transcriptional regulatory code by ide  ...[more]

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