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ProteomicsCold-induced phosphorylational sites analysis of protein kinase OST1
For the analysis of phosphorylation sites of OST1, 8 g pSuper:OST1-Myc plants materials, treated with 4oC for 0 or 2 h, were used for protein extraction and immunoprecipitated with anti-Myc agarose. Then OST1-Myc proteins were eluted with 0.1 M glycine solution (pH 2.8) and mixed with 1 M Tris solut...
ORGANISM(S): Arabidopsis Thaliana 
2020-11-16 | PXD022539 |
ProteomicsProteins raw data using CT-1 in MDA-MB-231
The MDA-MB-231 cells were treated with CT-1 for 24 h. Then the cells were collected for proteomic analysis. SDT buffer was used for sample lysis and protein extraction. Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described by Matthias Mann...
ORGANISM(S): Homo Sapiens 
2024-10-30 | PXD057337 |
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