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GenomicsA unique regulatory phase of DNA methylation in the early mammalian embryo
DNA methylation is highly dynamic during mammalian embryogenesis. It is broadly accepted that the paternal genome is actively depleted of global cytosine methylation at fertilization, followed by passive depletion that reaches a minimum at the blastocyst stage. However, this model is based on limit...
ORGANISM(S): Mus musculus 
2012-03-28 | E-GEOD-34864 | biostudies-arrayexpress
GenomicsGenome-scale DNA methylation maps of pluripotent and differentiated cells
We report the generation and analysis of genome-scale DNA methylation profiles at nucleotide resolution in mammalian cells. Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps covering the vast majority of C...
ORGANISM(S): Mus musculus 
2008-07-06 | E-GEOD-11034 | biostudies-arrayexpress
GenomicsSequential ChIP-bisulfite sequencing enables direct genome-scale investigation of chromatin and DNA methylation cross-talk
Cross-talk between DNA methylation and histone modifications drives the establishment of composite epigenetic signatures and is traditionally studied using correlative rather than direct approaches. Here we present sequential ChIP-bisulfite-sequencing (ChIP- BS-seq) as an approach to quantitatively ...
ORGANISM(S): Homo sapiens 
2012-03-27 | E-GEOD-28254 | biostudies-arrayexpress
TranscriptomicsCellular source and mechanisms of high transcriptome complexity in the mammalian testis (RNA-Seq cells)
Understanding the extent of genomic transcription and its functional relevance is a central goal in genomics research. However, detailed genome€wide investigations of transcriptome complexities in major mammalian organs  and  their  underlying  cellular sources, transcriptional mechanisms, and func...
ORGANISM(S): Mus musculus 
2013-06-20 | E-GEOD-43717 | biostudies-arrayexpress
TranscriptomicsCellular source and mechanisms of high transcriptome complexity in the mammalian testis (RNA-Seq organs)
Understanding  the  extent  of  genomic  transcription  and  its  functional  relevance  is  a  central  goal  in  genomics research. However, detailed genome‐wide investigations of transcriptome complexities in major mammalian organs  and  their  underlying  cellular  sources,  transcriptional  mec...
ORGANISM(S): Mus musculus 
2013-06-20 | E-GEOD-43721 | biostudies-arrayexpress
Genomicsde novo DNA Methylation Balances Hematopoietic Stem Cell Self-Renewal and Differentiation
Cytosine methylation is an epigenetic mark usually associated with gene repression. Despite a requirement for de novo DNA methylation for differentiation of embryonic stem cells, its role in somatic stem cells is unknown. Using conditional ablation, we show that loss of either, or both, Dnmt3a or Dn...
ORGANISM(S): Mus musculus 
2011-09-30 | E-GEOD-27322 | biostudies-arrayexpress
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