To identify SUMO-modified proteins and determine the potential SUMO-specific protease target, we first transformed a Strep-VdSUMO construct into VdΔulpb to create the VdΔulpb/Strep-SUMO strain for immunoprecipitation with anti-Strep. An anti-Strep-IPed sample was used for mass spectrometry (MS).
ORGANISM(S): Verticillium dahliae VdLs.17
