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TranscriptomicsCells pretreated with NaCl have an altered gene expression in response to H2O2.
BY4741 (S288c haploid) cells have different gene expression in response to 0.4mM H2O2 when pretreated with 0.7M NaCl compared to cells that are not pretreated with NaCl (mock cells). BY4741 cells were grown in log phase for 3 doublings (t0) and then the genomic expression of mock cells to 0.4mM H2O2...
ORGANISM(S): Saccharomyces cerevisiae 
2011-09-17 | E-GEOD-32196 | biostudies-arrayexpress
TranscriptomicsAn Ustilago maydis gene involved in H2O2 detoxification is required for virulence
The fungus Ustilago maydis is a biotrophic pathogen of corn. In its genome we have identified an ortholog of YAP1 from Saccharomyces cerevisae which regulates the oxidative stress response in this organism. yap1 mutants of U. maydis displayed higher sensitivity to H2O2 than wild type cells and their...
ORGANISM(S): Ustilago maydis 
2010-06-11 | E-GEOD-7518 | biostudies-arrayexpress
TranscriptomicsAtf1-mediated active gene response to H2O2 challenge.
Here we present the study on ChIP-chip mapping of the genomic binding sites for Sty1, Atf1, and the Atf1's binding partner Pcr1; the genome-wide transcriptional profiling of the atf1 and pcr1 strains in response to H2O2; and the phenotypic assessment of ~90 Atf1/Pcr1-bound or unbound genes for growt...
ORGANISM(S): Schizosaccharomyces pombe 
2010-07-19 | E-GEOD-13053 | biostudies-arrayexpress
TranscriptomicsEffect of H2O2 treatment on gene expression of islet derived hybrid cell of 1.1B4
We analyzed the comprehensive gene expression changes by H2O2 in pancreatic beta-cell derived 1.1B4 cells using Agilent microarray. The genes of 2903 H2O2 treated were up-regulated and 2283 genes were down-regulated compared with control sample. HMOX1, EGR1, IGF, IL8 and NR4A3 were strongly up-regul...
ORGANISM(S): Homo sapiens 
2016-06-16 | E-GEOD-83369 | biostudies-arrayexpress
ProteomicsProteome wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana
Protein carbonylation is a non-enzymatic and irreversible post-translational modification that occurs naturally in living organisms under the direct or indirect effect of reactive oxygen species (ROS). In this study, we analyzed proteins that are responsive to carbonylation by exogenous hydrogen per...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2022-10-17 | MSV000090540 | MassIVE
TranscriptomicsTranscription profiling of Arabidopsis shoots and roots in response to exogenous H2O2 with differential kinetics
Arabidopsis plants (Col-0) were hydroponically grown for 10 days at 22°C under 30~40 μE·m−2·s−1 and continuous light conditions. Treatment with 100 mM H2O2 or sterile distilled water as mock control by spraying for shoot treatments, or dipping for root treatments. Relationship between treated and ha...
ORGANISM(S): Arabidopsis thaliana 
2019-06-01 | E-MTAB-4961 | biostudies-arrayexpress
TranscriptomicsExpression profile for PA14 lasR rhlR double mutant with H2O2 stress
Quorum sensing controls the expression of multiple virulence factors. PA14 genes lasR and rhlR are necessary for quorum sensing via homoserine lactones. A PA14 lasR rhlR deficient mutant exhibits a reduced oxidative stress response. Here we conducted a microarray to determine oxidative stress resp...
ORGANISM(S): Pseudomonas aeruginosa 
2015-01-09 | E-GEOD-54245 | biostudies-arrayexpress
TranscriptomicsExpression profiling of small RNAs in control and H2O2-treated rice seedlings
We aimed to identify putative H2O2-regulated miRNAs expressed in rice seedlings under oxidative stress by using a deep sequencing approach developed by Solexa (Illumina). Two small RNA libraries were constructed from H2O2-treated and control rice seedlings, and more than five million small RNA seque...
ORGANISM(S): Oryza sativa 
2010-08-15 | E-GEOD-23217 | biostudies-arrayexpress
TranscriptomicsGene expression of hepatocyte cell line (HepG2) treated with H2O2 to induce senescence relative to control untreated cells
We used microarrays to identify a transcriptional signature of oxidative stress induced senescence in a hepatocyte cell line (HepG2) by globally assessing differential gene expression after treatment with 0.5mM of H2O2 for 60 minutes, compared to nontreated cells as a control. We performed genome-wi...
ORGANISM(S): Homo sapiens 
2015-01-01 | E-GEOD-47739 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1, H2O2 Stress Response
50mM H2O2 was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of H2O2 for 30 minutes, cultures were spun down and pellets were resuspended in same volume of GN101 media. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7610 | biostudies-arrayexpress
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