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TranscriptomicsSingle-cell RNAseq of HA-specific CD8 T cells isolated from the hypothalamus and the spleen of mice expressing the model antigen HA in orexinergic neurons
We previously generated the Orex-HA mouse model in which orexin-producing neurons residing in the hypothalamus selectively expressed a model self-antigen, namely hemagglutinin (HA) from the H1N1 influenza virus. In this model the adoptive transfer of in vitro activated HA-specific CD8 T cells leads ...
ORGANISM(S): Mus musculus 
2022-04-14 | E-MTAB-11416 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse HA specific T regulatory cells cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific T effector cells
Expanded HA-specific Thy-1.1 Tregs were cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific Teffs. After 5 days, Tregs were sorted by flow cytometry, based on the expression of the Thy-1.1 congeneic marker. Two biological duplicates were hybridized to the Sen...
ORGANISM(S): Mus musculus 
2009-09-08 | E-MTAB-146 | biostudies-arrayexpress
TranscriptomicsTranscription profiling time series of thymocytes from HNT-TCR transgenic mice injected with HA peptide to induce thymic negative selection
During T cell development in the thymus, negative selection eliminates autoreactive and potentially harmful T cells, leading to central tolerance. This process, involves specific apoptosis programs thought to be governed by the transcription factor Nur77. To analyze the genetic pathways involved in ...
ORGANISM(S): Mus musculus 
2011-06-20 | E-TABM-550 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human CD4 T Cells reveals Tumor and Self-Recognition Induce Distinct Transcriptional Profiles in Antigen-Specific CD4 T cells
Tumors express a wide variety of both mutated and non-mutated antigens. Whether these tumor antigens are broadly recognized as “self” or “foreign” by the immune system is currently unclear. Using an autochthonous prostate cancer model in which hemagglutinin (HA) is specifically expressed in ...
ORGANISM(S): Mus musculus 
2009-02-08 | E-GEOD-14662 | biostudies-arrayexpress
TranscriptomicsMiR-21 function in T-lymphocytes
T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral trans...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37213 | biostudies-arrayexpress
TranscriptomicsMurine CD8+ T cells: in vitro-derived vs. thymocytes
Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression prof...
ORGANISM(S): Mus musculus 
2012-09-05 | E-GEOD-37669 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-sequencing resolves a CD4+ T cell fate bifurcation
Single-cell RNA sequencing (scRNA-seq) was used to study the various transcriptional states of individual CD4+ T cells during blood-stage Plasmodium chabaudi infection in mice. This is an experimental model of malaria in which CD4+ T cells are essential for controlling parasite numbers, and which is...
ORGANISM(S): Mus musculus 
2017-03-21 | E-MTAB-4388 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of p56lck-deficient regulatory and memory T cells
Signaling through the T cell antigen receptor is essential for the formation of regulatory T (Treg) cells in the thymus and for their involvement in antigen-directed suppression of immune responses. Using a conditional null allele of the gene encoding p56Lck we show here that T cell antigen recepto...
ORGANISM(S): Mus musculus 
2010-05-22 | E-GEOD-13645 | biostudies-arrayexpress
TranscriptomicsGenome-wide transcriptional analyses of islet-specific CD4+ T cells identify Idd9 genes controlling diabetogenic T cell function
Analysis of gene expression levels in ex-vivo and p79-stimulated splenic CD4+ T cells. Total RNA obtained from ex-vivo CD4+TCRVβ4+ T cells from BDC-Idd9 mice compared to BDC mice and p79-stimulated CD4+TCRVβ4+ T cells from BDC-Idd9 mice compared to BDC mice.
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-64674 | biostudies-arrayexpress
TranscriptomicsExpression data from primary and secondary CD4 T cell effectors responding towards influenza A virus infection
How secondary CD4 T cell effectors, derived from resting memory cells, differ from primary cells, derived from naïve precursors, and how such differences impact recall responses to pathogens is unknown. We used microarrays to detail the global programme of gene expression underlying differences betw...
ORGANISM(S): Mus musculus 
2012-08-27 | E-GEOD-40230 | biostudies-arrayexpress
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