Single-cell RNA-sequencing resolves a CD4+ T cell fate bifurcation

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) was used to study the various transcriptional states of individual CD4+ T cells during blood-stage Plasmodium chabaudi infection in mice. This is an experimental model of malaria in which CD4+ T cells are essential for controlling parasite numbers, and which is characterized by concurrent development of Th1 and Tfh cells. We have used Plasmodium-specific TCR transgenic CD4+ T cells to minimise the effects of TCR diversity on Th fate decisions. Activated antigen-specific cells were studied at days 0, 2, 3, 4 and 7. In addition, dendritic cells and monocytes were studied at days 0 and 3. Cell lysis, RT and cDNA preamplification was performed using Fluidigm C1 system.

INSTRUMENT(S): Illumina HiSeq 2500, MoFlo XDP (Beckman Coulter) or a FACSAria II (Becton Dickinson), Fluidigm C1 autoprep system

ORGANISM(S): Mus musculus

SUBMITTER: Tapio Lonnberg 

PROVIDER: E-MTAB-4388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress