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TranscriptomicsUpregulation of miR-24 is associated with decreased DNA damage repair in highly differentiated CD8-positive T cells
microRNA transcript levels in CD8+ T-cells were profiled. Two experimental factors were considered: CD28 status (positive/negative) and Etoposide (10 M-5g/ml) treatment (etoposide/control).
ORGANISM(S): Homo sapiens 
2012-07-12 | E-MEXP-3307 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human fibroblasts from foreskin to determine changes in miRNA regulation with age
Foreskin samples were derived from young and old donors. Total RNA was extracted and used for hybridization to microRNA microarrays. From 4 young and 4 old donors, differential expression of microRNAs was analyzed using R/Bioconductor and linear models
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2459 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of human umbical vein endothelial cells (HUVEC) using young (replicating) cells and senescent cells
From two donors of human umbilical vein endothelial cells, in vitro cell lines were established. Both cell lines were grown in vitro until irreversible growth arrest was observed (replicative senescence). Total RNA from young (replicating) cells as well as senescent cells was harvested and used for ...
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2425 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human miRNA expression in mesenchymal stem cells in young and old donors.
Mesenchymal stem cells were isolated from several donors of different age (young vs old), and total RNA was extracted. Samples were labeled with Cy3 and Cy5 dyes and hybridized in a looped design that allowed the calculation of differentially expressed miRNAs in MSCs isolated from healthy, aged indi...
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2455 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of human CD8 T cells - miR-17 and miR-19b to investigate down-regulatation in human aging
T cells from several blood donors were separated in populations corresponding to CD8+ CD28+ and CD8+ CD28- surface markers. Total RNA extracts from cells were hybridized to MRC LNA microarray. Contrasts of interest were CD8CD28- minus CD8CD28+ (replicative aging) and CD8CD28+ (old donors) minus CD8C...
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2398 | biostudies-arrayexpress
TranscriptomicsIdentification of microRNAs specific for high producer CHO cell lines
Mature microRNA levels were analyzed in 4 producing CHO cell lines expressing 2 structurally different recombinant proteins at low and high level as well as in 1 non-producing CHO cell line. The goal was to identify microRNAs that are involved in heterologous protein formation and secretion. 5 recom...
ORGANISM(S): Cricetulus griseus 
2014-08-04 | E-GEOD-57023 | biostudies-arrayexpress
TranscriptomicsOncomiR-21 triggers growth arrest of endothelial cells via a complex network involving p21 CDK2 CDC25A and NFIB
miRNA transcription in senescent human umbilical vein endothelial cells (HUVEC) compared to young HUVECs.
ORGANISM(S): Homo sapiens 
2013-01-17 | E-MEXP-3596 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of human dermal fibroblasts - miR-17 and miR-19b to investigate down-regulatation in human aging
Normal human fibroblasts were isolated from skin biopsies of two different healthy causcasian patients, a 41-years old female (HDF-1) and a 16-years old male (HDF-5). HDF-5 and HDF-1 cells were cultivated in DMEM/HAM’s F-12 medium (Biochrom KG, Berlin, Germany) supplemented with 10% fetal calf serum...
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2386 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human renal proximal tubular cells of young or senescent replicative age; or high or low reactive oxygen species.
Renal Proximal Tubular Cells of young / senescent replicative age or high / low reactive oxygen species content were profiled using an Affymetrix Chip Platform.
Sample classifications:
Young refers to the in vitro age of early passage cells.
Senescent indicates cells at the end of their i...
ORGANISM(S): Homo sapiens 
2011-01-15 | E-MEXP-2683 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of human renal tubular epithelial cells - miR-17 and miR-19b to investigate their down-regulatation in human aging
Renal Tubular Epithelial Cells (RPTEC) from two donors were cultivated in vitro until irreversible growth arrest was observed. Total RNA from young (replicating) as well as growth arrested cells was harvested and hybridized to LNA based microRNA microarrays using a looped design. After data processi...
ORGANISM(S): Homo sapiens 
2010-04-19 | E-MEXP-2393 | biostudies-arrayexpress
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