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TranscriptomicsMapping interactions for the TNIP2 hub protein
This experiment analyzes the set of RNAs copurifying with the protein TNIP2 (amino acids 196-346) HEK293 cells were transfected with constructs expressing either Halo tag (controls) or Halo-TNIP2 196-346. Total RNA was purified from an aliquot of the whole cell extract (Input samples). Halo-tagged p...
ORGANISM(S): Homo sapiens 
2016-10-24 | E-GEOD-79656 | biostudies-arrayexpress
UnknownSNAP-, CLIP- and Halo-tag labelling of budding yeast cells.
Not available
S-EPMC3808294 | biostudies-literature
UnknownA Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools.
Not available
S-EPMC11901542 | biostudies-literature
UnknownThe Cation-π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification.
Not available
S-EPMC5362743 | biostudies-literature
TranscriptomicsControlling for gene expression changes in transcription factor protein networks.
The development of affinity purification technologies together with mass spectrometric analyses of the purified protein mixtures (AP-MS) has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactio...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-GEOD-57027 | biostudies-arrayexpress
TranscriptomicsAnalysis of the expression of Ataxin-2-target genes
We recently identified the RNA targets of Ataxin-2 by using PAR-CLIP. To elucidate the functional role of Ataxin-2 on target-binding, we have knocked down or overexpressed Ataxin-2 in HEK293T cells and extracted RNAs 48 hrs after transfection. Then, these total RNAs were subjected to whole transcrip...
ORGANISM(S): Homo sapiens 
2014-06-20 | E-GEOD-53189 | biostudies-arrayexpress
GenomicsE-GEOD-57027
Transcription profiling by high throughput sequencing of HEK293 cells expressing Halo-RelA, Halo-NFkB1, or Halo tag alone
2025-01-24 | E-GEOD-57027 | ExpressionAtlas
ProteomicsAP-MS analyses of Halo-SAP25 transiently expressed in 293T cells.
Different versions (full length and various mutants) of SAP25 tagged with an N-terminal Halo affinity tag were expressed in 293T cells. The resulting lysates were used to capture SAP25 associated proteins. Purified protein complexes were identified by AP-MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-01 | MSV000093553 | MassIVE
UnknownThe physiological impact of an N-terminal Halo-tag on glucose-dependent insulinotropic polypeptide receptor function in mice.
Not available
S-EPMC11885072 | biostudies-literature
ProteomicsAP-MS analyses of Halo- Sin3 complex subunits stably expressed in 293T cells.
Sin3 complex subunits tagged with an N-terminal Halo affinity tag were stably expressed in Flp-In-293 cells. The resulting lysates were used to capture Sin3 associated proteins. Purified protein complexes were identified by AP-MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-05 | MSV000093576 | MassIVE
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