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Little is known about the proteolytic turnover of mitochondrial proteins under static growth conditions. Combining dynamic isotope labeling and mass spectrometry in yeast cells, we found an exceptionally high turnover for the NADH dehydrogenase Nde1. This homolog of the mammalian apoptosis inducing ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-06 | PXD012531 | Pride
Chlamydomonas wild type CC 4533 and lpa2 mutant were grown in TAP medium at 25°C and ~30 µmol photons m^-2 s^-1 on a rotary shaker. Protein complexes of three wild type (wt) and three lpa2 (mut) samples were separated by BN-PAGE and cut into 36 slices. Subsequent mass spectrometry enables the analys...
ORGANISM(S): Chlamydomonas reinhardtii 
2021-09-28 | PXD023443 | Pride
The productivity of plants and microalgae needs to be increased to feed the growing world population and to promote the development of a low-carbon economy. This goal can be achieved by improving photosynthesis via genetic engineering. In this study, we have employed the Modular Cloning strategy to ...
ORGANISM(S): Chlamydomonas reinhardtii 
2021-02-11 | PXD018833 | Pride
Thiol-based redox regulation is a crucial post-translational mechanism to acclimate plants to changing light availability. Here, we conduct a biotin-switch-based redox proteomics study to systematically investigate dynamics of the thiol-redox network in response to temporal changes in light availabi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-29 | PXD043914 | Pride
Chlamydomonas wild type CC 4533 and psb28 mutant were grown in TAP medium at 25°C and ~30 µmol photons m^-2 s^-1 on a rotary shaker. Protein complexes of three wild type (wt) and three psb28 (mut) samples were separated by BN-PAGE and cut into 36 slices. Subsequent mass spectrometry enables the anal...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-05-08 | PXD023478 | Pride
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