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ProteomicsStoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach
Spores of Bacillus cereus pose a threat to food safety due to their high resistance to the heat or acid treatments commonly used to make food microbially safe. As spores survive these treatments and later resume growth either on foodstuffs or, after ingestion, upon entering the gut they are capable ...
ORGANISM(S): Bacillus cereus 
2018-10-26 | PXD003569 | Pride
Bacillus cereus Qconcat Quantitative proteomics Spores 
ProteomicsA Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)
In the present study, we developed a novel targeted proteomics approach for quantification of allele-specific protein expression (ASPE) based on scheduled high resolution multiple reaction monitoring (sMRM-HR) with a heavy stable isotope-labeled quantitative concatamer (QconCAT) internal protein sta...
ORGANISM(S): Homo sapiens (Human) 
2018-08-28 | PXD008788 | Pride
Human Allele-specific protein expression Liver 
ProteomicsHybrid QconCAT-based Targeted Absolute and Data-independent Acquisition-based Label-free Quantification Enables In-depth Proteomic Characterization of Wheat ATI Extracts
In this study, we present a novel hybrid data-independent acquisition LC-MS approach, combining QconCAT technology with short microflow LC gradients and DIA and apply the method towards the quantitative proteome analysis of ATI extracts from wheat flour. The novel method is fast, robust and reproduc...
ORGANISM(S): Triticum aestivum (Wheat) 
2021-04-22 | PXD021381 | Pride
Wheat Dia Data-independent acquisition Qconcat Absolute quantification Microflow lc-ms Ati Triticum Amylase-trypsin inhibitor 
ProteomicsDecoding the stoichiometric composition and organization of bacterial metabolosomes C4PR_LIV
Self-assembly of proteins into complexes with defined stoichiometry and organization is fundamental to the structure and functionality of many molecular machines in biology. Some enteric bacteria including Salmonella have evolved the propanediol-utilizing microcompartment (Pdu MCP), a specialized pr...
ORGANISM(S): Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Bacteria 
2020-05-11 | PXD015111 | Pride
Pdu mcp Qconcat Propanediol-utilising microcompartment Salmonella 
ProteomicsMEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATS for Large Scale Absolute Proteome Quantification C4PR_LIV
A major challenge in proteomics is the absolute accurate quantification of large numbers of proteins. QconCATs, artificial proteins that are concatenations of multiple standard peptides, are well established as an efficient means to generate standards for proteome quantification. Previously, QconCAT...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-10-27 | PXD007949 | Pride
Qconcat meercat 
UnknownConstruction of a la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins.
Not available
S-EPMC8425055 | biostudies-literature
UnknownQuantitation of Vacuolar Sugar Transporter Abundance Changes Using QconCAT Synthtetic Peptides.
Not available
S-EPMC4828444 | biostudies-literature
ProteomicsHybrid label-free quantification proteomics and QconCAT-assisted quantification of amylase-trypsin inhibitors from flours of 150 wheat varieties (3 field locations) by LC-MSE
Wheat (Triticum aestivum ssp. aestivum) contributes to 20% of the human protein supply, delivers essential amino acids and is of fundamental importance for bread and pasta quality. Wheat proteins are also involved in adverse human reactions like celiac disease, wheat allergy and the non-celiac wheat...
ORGANISM(S): Triticum aestivum (Wheat) 
2021-09-10 | PXD023654 | Pride
Mse Breeding Amylase-trypsin inhibitors (atis) Synapt g2-s Proteomics Wheat varieties Qconcat Label-free quantification (lfq) Non-celiac wheat sensitivity (ncws) Data-independent acquisition (dia) 
ProteomicsSynthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification C4PR_LIV
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of ...
ORGANISM(S): Escherichia coli 
2021-11-15 | PXD027521 | Pride
Synthetic biology Qconcat Quantitative proteomics Loop assembly 
ProteomicsStoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system
The multienzyme glycine cleavage system (GCS) converts glycine and tetrahydrofolate to the one-carbon compound 5,10-methylenetetrahydrofolate, which is of vital importance for most if not all organisms. Photorespiring plant mitochondria contain very high levels of GCS proteins organized as a fragile...
ORGANISM(S): Pisum sativum (Garden pea) Arabidopsis thaliana (Mouse-ear cress) 
2020-04-23 | PXD018211 | Pride
Arabidopsis Pea Quantification Stoichiometry Qconcat Glycine decarboxylase complex 
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