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Immunotherapy has revolutionized cancer treatment, yet many patients do not respond. Here, we profile the proteome and phosphoproteome of clinical pre-treatment samples from 73 esophageal squamous cell carcinoma (ESCC) patients undergoing anti-programmed death 1 (PD1) immunotherapy.
ORGANISM(S): Homo Sapiens 
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
2023-06-12 | PXD033812 |
The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detec...
ORGANISM(S): Homo Sapiens 
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