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The widespread adoption of single-cell proteomics (SCP) in biology has been limited by complex workflows and reliance on specialized instrumentation. Here we present EasySCP, a high-throughput and lossless method that integrates FACS-based single-cell sorting, an all-in-one, single-step digestion pr...
ORGANISM(S): Homo Sapiens Mus Musculus 
2025-10-30 | PXD070083 |
Histone PTM bottom-up profiling of Halo-WDR76 or Halo-SPIN1 Enriched or total histones (w Propionylation; no spike-in peptide) Supplementry data for WDR76-SPIN1 SCAP Analysis
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-04-15 | MSV000094548 | MassIVE
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits Halo-WDR76. Purification was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. Control purification was performed on bl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086849 | MassIVE
Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stab...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-11 | MSV000086846 | MassIVE
Halo purified WDR76 from a stable cell line of 293FRT in WDR76 KO background. DSSO crosslinking performed in eluted samples. Data collected using ms1/ms2/ms3 method. Data searched using Proteomics Discoverer 2.4 with XlinkX.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-09 | MSV000086828 | MassIVE
SCAP-XL of Halo-WDR76 and SNAP-SPIN1 from stable cell line in WDR76 KO background. Serial Capture Affinity Purification (SCAP) was performed on whole cell extract of a stable cell line of 293FRT cells in WDR76 KO background. DSSO crosslinked in E2 sample. Data was acquired using a ms1/ms2/ms3 method...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-22 | MSV000086749 | MassIVE
Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cel...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-03 | MSV000086802 | MassIVE
Identification of chemotherapy-treated B16-F10 tumor membrane proteins
ORGANISM(S): Mus Musculus 
2024-08-28 | PXD055424 |
Cell membrane protein-protein interactions (PPIs) play pivotal roles in maintaining cellular homeostasis and physiological balance. Deciphering these interactions is crucial for understanding disease mechanisms and identifying therapeutic targets. While proximity labeling technologies have revolutio...
ORGANISM(S): Homo Sapiens 
2026-02-27 | PXD074981 |
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