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GenomicsGenome-wide maps of GATA1 occupany in induced G1E ER4 cell line.
we mapped the locations of DNA segments occupied by GATA1 using chromatin immunoprecipitation (ChIP). We have produced genome-wide GATA1 ChIP datasets after restoration and activation in G1E-ER4 cells. we employed the sequence census methodology of ChIP-seq , using Illumina GA2 technology to produ...
ORGANISM(S): Mus musculus 
2009-09-19 | E-GEOD-18164 | biostudies-arrayexpress
TranscriptomicsExpression data from trisomy 21 and euploid induced pluripotent stem cell hematopoietic progenitors
We modeled human Trisomy 21 primitive hematopoiesis using induced pluripotent stem cells (iPSCs). Primitive multipotent progenitor populations generated from Trisomy 21 iPSCs showed normal proliferative capacity and megakaryocyte production, enhanced erythropoiesis and reduced myeloid development c...
ORGANISM(S): Homo sapiens 
2012-09-24 | E-GEOD-35561 | biostudies-arrayexpress
GenomicsIdentification of Biologically Relevant Enhancers in Human Erythroid Cells [ChIP-Seq]
Identification of cell-type specific enhancers is important for understanding the regulation of programs controlling cellular development and differentiation. Enhancers are typically marked by the co-transcriptional activator protein p300 or by groups of cell-expressed transcription factors. We hypo...
ORGANISM(S): Homo sapiens 
2013-01-28 | E-GEOD-43625 | biostudies-arrayexpress
TranscriptomicsIdentification of Biologically Relevant Enhancers in Human Erythroid Cells [Illumina BeadArray]
Identification of cell-type specific enhancers is important for understanding the regulation of programs controlling cellular development and differentiation. Enhancers are typically marked by the co-transcriptional activator protein p300 or by groups of cell-expressed transcription factors. We hypo...
ORGANISM(S): Homo sapiens 
2013-01-28 | E-GEOD-43624 | biostudies-arrayexpress
TranscriptomicsPluripotent stem cells reveal novel erythroid activities of the GATA1 N-terminus
This SuperSeries is composed of the SubSeries listed below. Germline GATA1 mutations resulting in the production of an amino-truncated protein termed GATA1s (for M-bM-^@M-^\shortM-bM-^@M-^]) cause congenital hypoplastic anemia. Similar somatic mutations promote transient myeloproliferative disease ...
ORGANISM(S): Mus musculus 
2015-01-26 | E-GEOD-61933 | biostudies-arrayexpress
GenomicsGenome-wide ChIP-Seq reveals a dramatic shift in the binding of the transcription factor erythroid Kruppel-like factor during erythrocyte differentiation
Erythropoiesis is dependent on the activity of transcription factors, including the erythroid-specific erythroid Kruppel-like factor (EKLF). ChIP followed by massively parallel sequencing (ChIP-Seq) is a powerful, unbiased method to map transfactor occupancy. We used ChIP-Seq to study the interactom...
ORGANISM(S): Mus musculus 
2013-06-17 | E-GEOD-48020 | biostudies-arrayexpress
TranscriptomicsLong noncoding RNA expression in several hematopoietic progenitor and differentiated cell populations using a custom Agilent microarray
Mammals express thousands of long noncoding (lnc) RNAs, a few of which are shown to function in tissue development. However, the entire repertoire of lncRNAs and the extent to which they regulate biological processes in different tissues and species are not defined. Indeed, most lncRNAs are not cons...
ORGANISM(S): Mus musculus 
2014-02-20 | E-GEOD-51892 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of mouse Gata1- megakaryocyte-erythroid progenitors (G1MEs) expressing one of the two isoforms of GATA1: full-length (GATA1fl) or an amino-truncated form of GATA1 (GATA1s).
We transduced mouse Gata1- megakaryocyte-erythroid progenitors with MIGRI-GFP vector expressing GATA1fl or GATA1s cDNAs. GFP-positive cells expressing one of the two isoforms of GATA1 were isolated by FACS 42 hours following transduction and used for microarray transcriptome analysis. At this time p...
ORGANISM(S): Mus musculus 
2015-01-26 | E-GEOD-62879 | biostudies-arrayexpress
GenomicsGenome-wide maps of epigenetic features in G1E model and in mouse primary erythroblasts.
Interplays among lineage specific nuclear proteins, chromatin modifying enzymes and the basal transcription machinery govern cellular differentiation, but their dynamics of actions and coordination with transcriptional control are not fully understood. Alterations in chromatin structure appear to es...
ORGANISM(S): Mus musculus 
2011-07-05 | E-GEOD-30142 | biostudies-arrayexpress
GenomicsGenome-wide binding analysis of GATA1fl and GATA1s in G1ME cells.
We used chromatin immunoprecipitation sequencing (ChIP-seq) to compare genome-wide binding profiles of GATA1fl and GATA1s in G1ME cells, which are immortalized, developmentally arrested megakaryocyte-erythroid progenitors (MEPs) derived from in vitro differentiation of murine Gata1- ES cells. Althou...
ORGANISM(S): Mus musculus 
2015-01-26 | E-GEOD-61932 | biostudies-arrayexpress
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