This study aimed to analyze ductal and squamous marker expression in pancreatic ductal adenocarcinoma (PDAC) cell lines under two-dimensional (2D) and three-dimensional (3D) culture conditions. Eight PDAC cell lines were examined using nanoLC-MS/MS-based proteomics.
Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development. We applied proteomic analysis to human brainstem organoids which were developed by a novel method.