Sort   by:  
 Page size 
ProteomicsHost Cell Protein Quantification Workflow Using Optimized Standards combined with Data-Independent Acquisition Mass Spectrometry
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-07-20 | PXD029305 | Pride
Hcp profiler Protein quantification Data validation. Data-independent acquisition (dia) Host cell proteins (hcps) 
ProteomicsProspective ICH Q2(R2)-aligned validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics
This dataset contains raw and processed LC–MS/MS data generated for a prospective validation of label-free untargeted proteomics for host cell protein (HCP) quantification in biotherapeutic matrices. A stable isotope-labeled CHO whole-proteome standard was spiked into the NIST monoclonal antibody re...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD075694 | Pride
Host cell proteins Hcp quantification Biotherapeutics Timstof Analytical validation Untargeted proteomics Ddapasef Ich q2(r2) Lc–ms/ms 
ProteomicsOptimized sample preparation, data processing of data-independent acquisition methods for the robust quantification of trace-level host cell protein impurities in drug products
In this study, we developed powerful and reproducible MS-based analytical workflows coupling optimized and efficient sample preparations, library-free DIA acquisition method and stringent validation criteria. The performances of several preparation protocols and DIA versus classical DDA were evaluat...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-10-19 | PXD019668 | Pride
Host cell proteins (hcp) Quantification Data-independent analysis (dia) Mass spectrometry Sample preparation 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039582 | Pride
Data-independent analysis (dia) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039585 | Pride
Faims Data-dependent analysis (dda) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsA Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development
There is a growing industry and regulatory need to detect host cell protein (HCP) impurities in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safety, and efficacy, even at low levels.1-6 In some cases, regulatory agencies require the identification as we...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-02 | PXD018427 | Pride
Monoclonal antibody (mab) Drug product (dp) Evosepone Drug substance (ds) Host cell proteins (hcps) Lc-ms/ms 
ProteomicsTargeted Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions
Host cell protein (HCP) impurities are considered a critical quality attribute of biopharmaceuticals because of their potential to compromise safety and efficacy, and LC/MS-based analytical methods have been developed to identify and quantify individual proteins instead of employing enzyme-linked im...
ORGANISM(S): Cricetulus Griseus 
2025-05-02 | PXD053135 | JPOST Repository
UnknownScout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices.
Not available
S-EPMC12921893 | biostudies-literature
UnknownHost cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry.
Not available
S-EPMC10257144 | biostudies-literature
ProteomicsDefinitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework
Host cell proteins (HCPs) are critical quality attributes in biotherapeutics that require accurate, specific, and comprehensive quantification. Mass spectrometry (MS)-based workflows are increasingly adopted to overcome the coverage and specificity limitations of immunoassays. This study benchmarks ...
ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029) 
MSV000098998 | MassIVE
Sort   by:  
 Page size