Most of pre-diabetes were eventually progress to diabetes, thereby profiling of prediabetic metabolic disorders may be an effective targeted preventive measure. We aimed to elucidate internal motivation of the progression of pre-diabetes to type 2 diabetes mellitus (T2DM) from a metabolic perspec...
Objective: This study aimed to explore the mechanisms of early-onset T2DM by analyzing proteomic changes at different stages of glucose metabolism. Methods: A total of 40 individuals undergoing routine physical health examinations between December 2016 and April 2017 were enrolled. Subjects were di...
Vitamin D (VD) has a coordinated immune function. Decreased VD levels are closely related to the occurrence of post-COVID-19 Conditions (PCC), but the specific mechanism is still unclear. Methods: We collected 50 PCC patient serum samples and divided them into three groups by different VD levels: VD...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-s...
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
During translation, protein quality control systems play an active role in ensuring the proper folding and assembly of proteins. To investigate the auxiliary factors that facilitate the folding and assembly of METTL3-METTL14, we employed proximity labeling technology using the split APEX2 system
